GGA1 (NM_013365) Human 3' UTR Clone

CAT#: SC210725

3' UTR clone of golgi associated gamma adaptin ear containing ARF binding protein 1 (GGA1) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: GGA1
• ACCN: NM_013365
• Insert Size: 883 bp
• Sequence Data:
  Insert Sequence

  >SC210725 3’UTR clone of NM_013365
  The sequence shown below is from the reference sequence of NM_013365. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CCCCCACCTGAAACCTGGGGTAGCCTCTAGAACAGAGGGGCTGGGGAGAGGAAGGGGCAGAGGGACCGG
  TCACTGTCCAGCCTGGAGGGAGGCATTGGTGGCCAAGGACACCCTTTGTTGCCCATGGCCATTCACCCC
  CAGGCCTGGTGCTTCTCCCCACACCCCTGTAGGCCTCAAGTGACTCTTCCCCCTCCTGCTCCGGCCCCG
  CCCCTGCTGAGCCAAACCCAGTAGGAGGCTGGGCCTGGGTTTGTGCCGCTGGGGTCTCCATCACCGGGA
  CCTGGAGAGGGAGGGGCTGTGTAGCCTTGGAAGAACTTGGGTCATGGGGAGGAAGCACAGCTGTTGGGG
  AAGGGCCAGGACCTCAGGCCCAGCCCCAACCCCAGCTGGGGTGGGGTCTTCCCCACCTGTCTCTTATGC
  CTTATGGGAAGGCCCAGCCATAACTCGGGGGCCATGCTGGAGCTGGGGACCAGCTTAGGCCTCCTCCAT
  AGGAACCCAGTGACTGGGGGGTGACGCCTACACCCCCAGCTATTTGCACTCTGGTGTGTGGTTTGACTC
  TGCTTTTCTTCCGGATTGGCCCTGTGGTCACAGCCTCAGGGGGCCAGGCTGGGGGAACCTCACCTGGCC
  CGTACTCCTGGGGGTTTCCCTTTGCCATTGGGCCCCCTGAGGGACTGTGGGGGCTCAAGGGTAATGCCA
  GAGGCCCATGGCCCCAGCGAGGGGCTGTGGGGCACCTAGAGTTCTCGGTGTGTCTCCTTCATTCATTGG
  CCTCTGCTGGGGCCTCCTATGGGTGTCTTACGTCTGTCCATCCATCTGTCCGTGGTCAGAAGTGGGGTC
  AGTGTGTGAGTGAGAGCAGGAGTATTTATGAAAATAAAACGTCGTTTTTCCTGGA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_013365.5
• Summary: This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) protein family. Members of this family are ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Locus ID: 26088
• MW: 31.2