CDH12 (NM_004061) Human 3' UTR Clone

CAT#: SC210664

3' UTR clone of cadherin 12 type 2 (N-cadherin 2) (CDH12) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CDH12
• Synonyms: CDHB
• ACCN: NM_004061
• Insert Size: 1094 bp
• Sequence Data:
  Insert Sequence

  >SC210664 3’UTR clone of NM_004061
  The sequence shown below is from the reference sequence of NM_004061. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GAGAGTTATAACCCTGATAAAGTCACTTAAGGGAGTCGTGGAGGCTAAAATACAACCGAGAGGGGAGAT
  TTTTAAAAAAGAAAAAGAAACACAAATATTAATAGAAATCTCTCTCACACACACACACACACACAAACA
  CACACACACACACACACACTCTGGGACAAGATTCCTTTGATTACCTGGTTTCTAGTAAGTTACTAAATT
  AATCATATTGTCAGTTTTGGTTTATTCTGCCAACAAGAGATACATCCTATAATGTGTACTTGCTTTGTT
  TTGTTGTTTTGTTTTTTCACAATCACACTGAGACAAGCTCAGGCCTATTCAATACAATTTACTGACATG
  ACAACCTAGAACGAAGATAGCTATGTGGCATCACGGTGGAAGAGTGTTAGATTTTTCTTAATTCCACTA
  AAGTGCCTATTGAAACTCTTATCATTTAAAGTGGTGTACAGTACACTCTGCTGTGATGGTATTGCGGGA
  TTCAGAGATAAAGACATAAAACAAAGACACCGTCTTTATGTCAAGAAGCAATAGCGACACTCTGACTCT
  CCTCATTCCTTTTGAGAACAAAAGAAGAACACTTTCTGATCTCCACCTTCTTCTAATTCAAAAGGTTTT
  CTTTTTCTTTTTTAAACTGTATGCTAAAACGTATTTGTTTATCCTATCCTTTCAGAAAATTGAAATAAA
  TATGAAAATATCAAGTTCAATATGTAAGTCCCAAATTCTAGAAACAATGTTTGATCTTAACATTAGTTC
  ACATTAAAGCTGTTCATTAAAATGTTACTTTCTTTTCCAAAAAAAAAAGAAAGAAAAAAAAGAAAAAAT
  ATTCCTCTTTATAAAGAGAGGCCTCAGGGCTCAAATTACACATTAAAATAAATATTGGTTTTGCTTAAT
  ATTGTACTGGTGTGTGGAAAATTGGTTTTAACAGTCACCAGATTCAGCTTTTAGTCATAGATGTAATTT
  CAAGTTGTGTTTCTATGAAAATTCTTAGAAGAAAAAAGTGCCATTCATTTAAGCATGGAAAGAGAAATT
  CATTATACAGTTTCTCAGAGTTTCTTACAATCTAGTGATTCTTTGATGAGCAGCAAAAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004061.5
• Summary: This gene encodes a type II classical cadherin of the cadherin superfamily. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature cadherin protein. These integral membrane proteins mediate calcium-dependent cell-cell adhesion and are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a histidine-alanine-valine (HAV) cell adhesion recognition sequence specific to type I cadherins. This particular cadherin appears to be expressed specifically in the brain and its temporal pattern of expression would be consistent with a role during a critical period of neuronal development, perhaps specifically during synaptogenesis. [provided by RefSeq, Nov 2015]
• Locus ID: 1010
• MW: 42.7