DHODH (NM_001361) Human 3' UTR Clone

CAT#: SC210645

3' UTR clone of dihydroorotate dehydrogenase (DHODH) nuclear gene encoding mitochondrial protein for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: DHODH
• Synonyms: DHOdehase; POADS; URA1
• ACCN: NM_001361
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC210645 3’UTR clone of NM_001361
  The sequence shown below is from the reference sequence of NM_001361. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GATGCCATTGGAGCAGATCATCGGAGGTGAGGACAGCGTCTGACGGGAAGCCTGATCTGGAACCTTCCC
  AAGGACTCAGGCAAGCCTTTGTGGCTGGATCATGAGAGGAGGGACTCCATCTTGAGCCATGTCCCCCAG
  CCATGGCATGGCTGCACTGTAAACGCCAATCGGGGGGTCACCAGGATCAACCGCAGGCTTTCTTCAGTC
  CCTTGGTCAGACCATAAACTGCATTTTTGATTCTTTGTGGATTCAAACCCTAGGATCCATCAGTCTTGC
  AAGGACATTGAATATTAGGAGGAAAAAGTCATGGAAAAAATAAAGCCATTTAGAACCTGGGTTTCAACG
  CTAGCCCTTTCTGGTTTGCCATAGGCCCTGCCAAGATACTGCAGGTCCATCCAGGCCTCTGCTATCTGC
  ATCTGCAGTGGGCTTCCCAGGAACTTGACTGTCTTTCATTTGATCTTTATTTTTGTTTATTTAATATTT
  TAAACTTTATTTTAAAAATATTTCAAACATAAGGGCGGGGTGTGGTGGCTCATGCCTGTAATCCCAGCA
  CTTTGGGAGGCCGAGGCGGGCGGATCACCTGAGGCCAGGAGTTGGAGACCAGCCAGGCCACCATGGTGA
  AACCCTGTCTCTACCAAAAATACAAAAAATTAGCCAGATATTGTGGCAGGCACTTTTAATCCCAGCTAC
  TCGGGAGGCTGAGGCAGGAGAATTGCTTGAACCTGGGAGGCGGAGGTTGCAGTGAGCCAAGATTGCACC
  AGTGCACTCCAGCTTGGGCGACAGATCAAGACTCTGTCACACACAAAACAAACAAACAAATTTTCCCGT
  TTCTCTCTGTCCCTTTCTCTATAACATGGATAAAATATTCACGTCTTATGTATTTATTATTGCTGAGTC
  ATTGGTGCCTTATTCTTCAGTGTTTCAGTCTGTATCTCCTAAGCATCCTGATGTTAGTTTTTTGACTAT
  AAAATTGACCTGCATTCTTTACTAGCAATTCAAACGGTACAGCACTCTTGAAGTGTAAACATTCCTGTT
  CCTCCTCACCCCACTCTGCAGACATGCCTTTCTGTCTGTCCTCCCAGACTTTTCCCCTGCATAAAGATG
  TTCATTTTGTACATACACTCAGACATACATGTGGCTGTATTTTTTGTATACCGATTTCTGGATGGAATG
  CACACTGTTATCTGTTTAGTTACTTTATGTTTTCATACAAATGACATCATCCCACAAATAGAATTCTGC
  CATTTACTTTCTTCACTATTCTTATGTGGCATGGTTCATGCTGGGCCTCGGTCTTTTGAGTGGGGTCTG
  TTTACCCAGTCCCCTGTTGGTGATCACATCGCTAACTTCCGGCGTTTTCCTATCACCATCGGTGCCTCC
  GTGAACACCTTGTGAATGTGCCTGTGCCTTCGTTCTGGTGTCGGTGCAGAGCAGGGTCCAAGGGGCGTG
  ACAGCTGGGCTGAGGGGCTGGCATGTTTCACATTCTGATAGATAGAGCCAAGTTGCTCCTATCAGGTTT
  TCAAGCTGCCCATTTCCCACCGTGGAGACAGCAACAGAGGGGACATCCCCAGCTCCTCTGGCTCTGCCT
  GTTCAAAGCTACCCTGTGAGGCCTGAAGACAGAGCTGCCCTGAAGGCAGCTCATGTCCACTGAACACTG
  ACTGTGGGCTGGATGCTGTGCCAAGGGCTTGACGTTTCCTCTTTAAGGATGAGGAAACGGGCAGAGAAG
  CGGCTGATTGTCCAGTCCCCCTGCGTGGAGGCTGCTTGGCTGGGCTCGAGCCCAGCGGTGTGGTCATAG
  AGCATGTTGTGCTGAATGGCTCTGCCATGGACCAAGCCTTGGCTGCTCTAAAAGCCGGCTCATTGCCTT
  CTGCTTCTCCGGATTCGTTCCTTACTCCAGGAAGAGCAGTGCACTGAAGGAGAAATGGGAATGGGCAAA
  AGGAACCACATGCTGGCCAGACCCCTGCGGGGTCACTTTCCTCAGCCTGGGCTCCGACATGCCACGTC
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001361.5
• Summary: The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane. [provided by RefSeq, Jul 2008]
• Locus ID: 1723
• MW: 74.8