Carbohydrate sulfotransferase 1 (CHST1) (NM_003654) Human 3' UTR Clone

CAT#: SC210239

3' UTR clone of carbohydrate (keratan sulfate Gal-6) sulfotransferase 1 (CHST1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CHST1
• Synonyms: C6ST; GST-1; KS6ST; KSGal6ST; KSST
• ACCN: NM_003654
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC210239 3’UTR clone of NM_003654
  The sequence shown below is from the reference sequence of NM_003654. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GAGGAGCGGGACTTCCGCCCCTTCTCGTGACCCGGGCGGTGCGGGTGGGGGCGGGAGGCGCAAGGTGTC
  GGTTTTGATAAAATGGACCGTTTTTAACTGTTGCCTTATTAACCCCTCCCTCTCCCACCTCATCTTCGT
  GTCCTTCCTGCCCCCAGCTCACCCCACTCCCTTCTGCCCCTTTTTTGTCTCTGAAATTTGCACTACGTC
  TTGGACGGGAATCACTGGGGCAGAGGGCGCCTGAAGTAGGGTCCCGCCCCCCCCCCCCCCATTCAGACA
  CATGGATGTTGGGTCTCTGTGCGGACGGTGACAATGTTTACAAGCACCACATTTACACATCCACACACG
  CACACGGGCACTCGCGAGGCGACTTCTCAAGCTTTTGAATGGGTGAGTGGTCGGGTATCTAGTTTTTGC
  ACTGTCTTACTATTCAAGGTAAGAGGATACAAACAAGAGGACCACTTGTCTCTAATTTATGAATGGTGT
  CCATCCTTTCCCCATCCCTGCCTCCTGCCCCTGACGCCCATTTCCCCCCTTAGAGCAGCGAAACTGCCC
  CCTCCTGCCCGCCCTTGCCTGTCGGTGAGGCAGGTTTTTACTGTGAGGTGAACGTGGACCTGTTTCTGT
  TTCCAGTCTGTGGTGATGCTGTCTGTCTGTCTGAGTCTCGTGGCCGCCCCTGGACCAGTGATGACTGAT
  GAATCTTATGAGCTTCTGATTGATCTCGGGGTCCATCTGTGATATTTCTTTGTGCCAAAAAGAAAAAAA
  AAGAGTGGATCAGTTTGCTAAATGAACATTGAAATTGAAATGCTTTATCTGTGTTTTCTGTAAATAAAA
  GAGTGCAATAATCTCTGTGTGTGATTGCAGGACATTCTGAATGGGTACGAGAGGGCCTCAGCCGGGTCT
  GGGTGTTCCTAGCTTTGGGGAGGACAGCGAGAGAGAGGTGGAGGTGGGAATTAAATGACAAGTCTGCCT
  TTCAGAACTCTTGTCACCCTCAACACTGAGTTTAGTTTAGGTTTTTGTTTTGTTTTGTTTTGAGACAGA
  GTCTCGCTCTGTCACCCAGGCTGGAGTGCAATGGCGCCATCTCGGCTCACTGCAACCTCTGCCTCCCAG
  GTTCAAGCAGTTCTCCCGCCTCAGCCTCCCGAGTGACTGGGACTACAGGTGCACACTGCCACACCTGGC
  TATTTTTTTTTTTTTTTGGATTTTAGTAGAGACGGGGTTTCACCGTGTTGTCCAGGCTGGTCGCGAACT
  CCTGAGCTCTGGGCATTCCTCCTGCCTCAGCCTCCCAAAGTGCTGGGATTGCAGACTTGAGCCACCTCA
  CCCAGCCGTTTTTTTTTTCCTTCAGTCAGTTGAGACCGGGTAACTTATAGCCAGGGATAATGTTCAAAG
  TCTTTAACAAGTGGTATGGCTTGCTGAATCAGAGTAGCTGGAGGTAAGAATGACATCCTGGAATCCCTT
  CTTGTGCTAAATGTTACCCATTTAGTTGGTGGGTCTGGGTCTAGGATAGCCAGGGAGGGGGAAACTTCA
  AGGGGTGGGGTGCCTCAGGGCAGTGGCTGCTGACCCACAGACCTGGAAGTATTTGACTATTTGAATAAC
  TGGTACAGGTTTACTGGTGTCTACACTGGCAGAGAATCTGCTGTGCTTCCTCCCCTTCCCTTCGTGGCA
  ATCTAGCAGGTTGCTCCAGGCAGCTTCCCCAGCTCTTATTCCAAGAAAACCTGCCCCTGGGGTGGCTGC
  AGGTCACAGCTTTGTTGTCCTGGGACAAAGGAGTGTCTGGTGATTAGGCAGCCCCAGGGGTCCTAGCCA
  ACTGGAAGAAGGGCTTCCTGAGGAAACACAGTCCTGGGCTATTTTATATGTGTCAGGAGGATGTAGACA
  GTTCAACAGCTCTAGCAAATGCCTTAACTAATAGTGGCTTAATCAAGATAGGTGATTTTCTTTTGTAAA
  AAATTTTACTGGGGTAAAATAACAGAATGTAAAATTTACCATCTTAACCTTTTTAAGGTGTACCATTC
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003654.6
• Summary: This locus encodes a member of the keratin sulfotransferase family of proteins. The encoded enzyme catalyzes the sulfation of the proteoglycan keratin. [provided by RefSeq, Aug 2011]
• Locus ID: 8534
• MW: 74