HIPK3 (NM_005734) Human 3' UTR Clone

CAT#: SC210209

3' UTR clone of homeodomain interacting protein kinase 3 (HIPK3) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: HIPK3
• Synonyms: DYRK6; FIST3; PKY; YAK1
• ACCN: NM_005734
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC210209 3’UTR clone of NM_005734
  The sequence shown below is from the reference sequence of NM_005734. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  ACAAAACTCAGCCAGTATCCATATATGTGAAAAACAGTATATTGGGGAAGCTCAATGATACAAACATTT
  GATTAAAAATAAAAACATGGTATTTAATATTAGCCATGGCACAAGAAAATTATTTTTGAATCATGTAGA
  CTTGGGTGCAATTTAAACAACTTTGAGCTTTAAAAACTCACTTTTGATGTGTTTTGCACATTTGGTATA
  ACTTGTCTTTGGTCATGTTATCTTCTTATGTAGTAACTCTAGACAGGTGACTTATGGGAGCAGAAGTCC
  AGTTTTGCTCCTGCTATTTTTTATAAATTGCCTTCTAACTAGTGCAAGACACGTCTACATTTGGGAAGC
  CATTCTGTGTACAGACTTAGAGCAACAGATGCACATATGTCAGAATTACAGCATACAAGTGAATTGTAT
  TATCCGTGTCTTAGTGTATAAATGTTGGGTCACTTACCTAAGAAATTGAGCTATTGTTCTTTACATTTG
  CATGTGTCTTTTGCATGGGCAAAATGTTGCCTAGACTTTGCTCTTAAATGTTGTTCTAATAATCTCAGC
  TGCATTGTAAACCGTTCCTACACATAGTGCCTTAAATATTTGAGGTTGTTAATGTTATTACCTATATAT
  AAATGTTGAGGACTGCAGCACTTAAAATTCAGACCTACTATTTAGTTTCCTTTTGATAGCGTAATGTTC
  ATTTTTGTTTTTGTGTGGTATGATTTCAGGTAGTAGCTGTTTTTTTCCTTATTAAGAGGGCAGCATGTT
  TGCTATAGCTGAATTCTGCTGTCTGATTTTTCAGAATGATCTAGCTTCAAGAAAAGCAAGCAGTTAGTA
  GTGCTTAAGAAAAATTGATTCAGTATCTAATGGATAGTTGATAACTGTCACAGCACAGCATTTTATATA
  CTGTTAAGTGAAACTGCAATACAATCTAAGTTTATTTTGAGAGTGTTTGCTGTATAATTGGACTTAATA
  AAATGTTTAGAGGTACAGTAGGCATGACTTTGGGTAGATAATAAAATGCAAAATGCTCATTGATTCATG
  ATGTGGTTTTATCTTAGCTTGGGCAAACCATGCAGTATTTAATAAATAGTAGCAGAACTTTTACTATTG
  AAGCTTGAAAAGATGTGAGTTCTTTGTGTGCAATTTTTGATTTATGCATGTGAGAGGGTTTTTTTTTTT
  TTTAAGTATTTTTACATTGCAGTACTTGTTTTGCTTGTTGGTGATGTTTGCTTATTTAATACATTCCGT
  CAGGGACAGAAATTACATGCTTTTTTTTCTTTCTAGGAAGTGTGTTGAGTTCCCCCCTTCCCCGACATT
  TTTTTCTTTTTTGGGTGGTGGTGATGGTAGTGGTTATGTCTGTTTAAATGAAGTTGCTTTTACAGCACC
  AAAGACTTAATCATCCATTTCCTATATAAAGGTAGCTACTTTTTGCATAGACCTCAAGTATATTGTAGT
  GTAAAGGTGGAATTTAAGGAAAGGTATTAAATTGAGGCTGTGTTTTAGCTTACAGGCAAGTAATAAATT
  GTATCATTTATCTTGAATGTATCATAGATAAGCTGCTATATAACGATTGCCACTTCAGATAGCTGTGAA
  ATTAGGTGATTAACTAGTTGTTATTTAGCCTTCTAATTTCTGTATAAGTCTAATTACATGAATAGAAAT
  TGGGGTTTTGATTTTTTACTTTGCTTTTCTGTTTGGAGTGTCATTGTAACTACTGTATTGTAAATGGTG
  GAAAATAATTGCATTTGTTACTTTGGGGTGTGTTATTTGCATCAGTATTTTATCTCTATTAATGTTTGT
  GCTCATCACTGCATATTAAAAAAACTTGGATGTATCAGTGTAACTTAATTTTTTATTTTCATACTGGCA
  TTGTAGACACTTGAGAAAGCTGTATCTTGCAGGCTTGACTTAACTTTTTTCCTTAAAAATCTGGAATAT
  AATCTTACAGCATTTACTGGAATAAACAGTACAAAGCATTGGAGTGTAAAACTCTAGATGTTTTGGAT
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_005734.5
• Summary: Serine/threonine-protein kinase involved in transcription regulation, apoptosis and steroidogenic gene expression. Phosphorylates JUN and RUNX2. Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. The phosphorylation of NR5A1 activates SF1 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. In osteoblasts, supports transcription activation: phosphorylates RUNX2 that synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE).[UniProtKB/Swiss-Prot Function]
• Locus ID: 10114
• MW: 78.5