HIPK3 (NM_005734) Human 3' UTR Clone
CAT#: SC210209
3' UTR clone of homeodomain interacting protein kinase 3 (HIPK3) transcript variant 1 for miRNA target validation
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Specifications
Product Data | |
Vector | pMirTarget |
Species | Human |
Transfection Reporter | RFP |
Assay Reporter | Luciferase |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Symbol | HIPK3 |
Synonyms | DYRK6; FIST3; PKY; YAK1 |
ACCN | NM_005734 |
Insert Size | 2000 bp |
Sequence Data |
>SC210209 3’UTR clone of NM_005734
The sequence shown below is from the reference sequence of NM_005734. The complete sequence of this clone may contain minor differences, such as SNPs. Blue=Stop Codon Red=Cloning site GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC ACAAAACTCAGCCAGTATCCATATATGTGAAAAACAGTATATTGGGGAAGCTCAATGATACAAACATTT GATTAAAAATAAAAACATGGTATTTAATATTAGCCATGGCACAAGAAAATTATTTTTGAATCATGTAGA CTTGGGTGCAATTTAAACAACTTTGAGCTTTAAAAACTCACTTTTGATGTGTTTTGCACATTTGGTATA ACTTGTCTTTGGTCATGTTATCTTCTTATGTAGTAACTCTAGACAGGTGACTTATGGGAGCAGAAGTCC AGTTTTGCTCCTGCTATTTTTTATAAATTGCCTTCTAACTAGTGCAAGACACGTCTACATTTGGGAAGC CATTCTGTGTACAGACTTAGAGCAACAGATGCACATATGTCAGAATTACAGCATACAAGTGAATTGTAT TATCCGTGTCTTAGTGTATAAATGTTGGGTCACTTACCTAAGAAATTGAGCTATTGTTCTTTACATTTG CATGTGTCTTTTGCATGGGCAAAATGTTGCCTAGACTTTGCTCTTAAATGTTGTTCTAATAATCTCAGC TGCATTGTAAACCGTTCCTACACATAGTGCCTTAAATATTTGAGGTTGTTAATGTTATTACCTATATAT AAATGTTGAGGACTGCAGCACTTAAAATTCAGACCTACTATTTAGTTTCCTTTTGATAGCGTAATGTTC ATTTTTGTTTTTGTGTGGTATGATTTCAGGTAGTAGCTGTTTTTTTCCTTATTAAGAGGGCAGCATGTT TGCTATAGCTGAATTCTGCTGTCTGATTTTTCAGAATGATCTAGCTTCAAGAAAAGCAAGCAGTTAGTA GTGCTTAAGAAAAATTGATTCAGTATCTAATGGATAGTTGATAACTGTCACAGCACAGCATTTTATATA CTGTTAAGTGAAACTGCAATACAATCTAAGTTTATTTTGAGAGTGTTTGCTGTATAATTGGACTTAATA AAATGTTTAGAGGTACAGTAGGCATGACTTTGGGTAGATAATAAAATGCAAAATGCTCATTGATTCATG ATGTGGTTTTATCTTAGCTTGGGCAAACCATGCAGTATTTAATAAATAGTAGCAGAACTTTTACTATTG AAGCTTGAAAAGATGTGAGTTCTTTGTGTGCAATTTTTGATTTATGCATGTGAGAGGGTTTTTTTTTTT TTTAAGTATTTTTACATTGCAGTACTTGTTTTGCTTGTTGGTGATGTTTGCTTATTTAATACATTCCGT CAGGGACAGAAATTACATGCTTTTTTTTCTTTCTAGGAAGTGTGTTGAGTTCCCCCCTTCCCCGACATT TTTTTCTTTTTTGGGTGGTGGTGATGGTAGTGGTTATGTCTGTTTAAATGAAGTTGCTTTTACAGCACC AAAGACTTAATCATCCATTTCCTATATAAAGGTAGCTACTTTTTGCATAGACCTCAAGTATATTGTAGT GTAAAGGTGGAATTTAAGGAAAGGTATTAAATTGAGGCTGTGTTTTAGCTTACAGGCAAGTAATAAATT GTATCATTTATCTTGAATGTATCATAGATAAGCTGCTATATAACGATTGCCACTTCAGATAGCTGTGAA ATTAGGTGATTAACTAGTTGTTATTTAGCCTTCTAATTTCTGTATAAGTCTAATTACATGAATAGAAAT TGGGGTTTTGATTTTTTACTTTGCTTTTCTGTTTGGAGTGTCATTGTAACTACTGTATTGTAAATGGTG GAAAATAATTGCATTTGTTACTTTGGGGTGTGTTATTTGCATCAGTATTTTATCTCTATTAATGTTTGT GCTCATCACTGCATATTAAAAAAACTTGGATGTATCAGTGTAACTTAATTTTTTATTTTCATACTGGCA TTGTAGACACTTGAGAAAGCTGTATCTTGCAGGCTTGACTTAACTTTTTTCCTTAAAAATCTGGAATAT AATCTTACAGCATTTACTGGAATAAACAGTACAAAGCATTGGAGTGTAAAACTCTAGATGTTTTGGAT ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG |
Restriction Sites | SgfI-MluI |
OTI Disclaimer | Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs). |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. |
Reference Data | |
RefSeq | NM_005734.5 |
Summary | Serine/threonine-protein kinase involved in transcription regulation, apoptosis and steroidogenic gene expression. Phosphorylates JUN and RUNX2. Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. The phosphorylation of NR5A1 activates SF1 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. In osteoblasts, supports transcription activation: phosphorylates RUNX2 that synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE).[UniProtKB/Swiss-Prot Function] |
Locus ID | 10114 |
MW | 78.5 |
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