HIP55 (DBNL) (NM_001014436) Human 3' UTR Clone

CAT#: SC210152

3' UTR clone of drebrin-like (DBNL) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: DBNL
• Synonyms: ABP1; HIP-55; HIP55; SH3P7
• ACCN: NM_001014436
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC210152 3’UTR clone of NM_001014436
  The sequence shown below is from the reference sequence of NM_001014436. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CCTGCCAACTACGTGGAGCTCATTGAGTGAGGCTGAGGGCACATCTTGCCCTTCCCCTCTCAGACATGG
  CTTCCTTATTGCTGGAAGAGGAGGCCTGGGAGTTGACATTCAGCACTCTTCCAGGAATAGGACCCCCAG
  TGAGGATGAGGCCTCAGGGCTCCCTCCGGCTTGGCAGACTCAGCCTGTCACCCCAAATGCAGCAATGGC
  CTGGTGATTCCCACACATCCTTCCTGCATCCCCCGACCCTCCCAGACAGCTTGGCTCTTGCCCCTGACA
  GGATACTGAGCCAAGCCCTGCCTGTGGCCAAGCCCTGAGTGGCCACTGCCAAGCTGCGGGGAAGGGTCC
  TGAGCAGGGGCATCTGGGAGGCTCTGGCTGCCTTCTGCATTTATTTGCCTTTTTTCTTTTTCTCTTGCT
  TCTAAGGGGTGGTGGCCACCACTGTTTAGAATGACCCTTGGGAACAGTGAACGTAGAGAATTGTTTTTA
  GCAGAGTTTGTGACCAAAGTCAGAGTGGATCATGGTGGTTTGGCAGCAGGGAATTTGTCTTGTTGGAGC
  CTGCTCTGTGCTCCCCACTCCATTTCTCTGTCCCTCTGCCTGGGCTATGGGAAGTGGGGATGCAGATGG
  CCAAGCTCCCACCCTGGGTATTCAAAAACGGCAGACACAACATGTTCCTCCACGCGGCTCACTCGATGC
  CTGCAGGCCCCAGTGTGTGCCTCAACTGATTCTGACTTCAGGAAAAGTAACACAGAGTGGCCTTGGCCT
  GTTGTCTTCCCCTATTTTCTGTCCCAGCTCATCCGTGTCTCTGAAGAACAAATATGCTTTTGGACCACG
  AATTCCCAGTTTGGTCTACACAGGCTCGCAGCTCTCACCTGTCCTGCTGTAGCCTGAATGCTCCCATGT
  GGGAATAGCACCCCACCACCTGCCCAGTGTCTGCCTCTGCTCCGTGTCTGGGGTGAGCACCCTCACCCT
  GAGACTGTGGCTGATGATGATCAGCCACCAGGAAGAGGCTAAATGTTAAGATGGGACTGAAGTTGGAGC
  CTTCCTGCGTTCCAGTTCCTCTAGGAGAGGCCAAGGGCTGCCCTGCTCCCAGCTTCAGGGAGCCTCTGC
  CTACATGGGATGGCCCACTGTGTGGCGTCAGGTACCTGGCACCCACTGAGCATCCGGGGAATTAGGTCC
  CTCCTGTGCCCCAGAGCTGCTAGGCAGACTGCTAGGCGGTTGACTCAAGGTGTTACCTGCTAGTGCCTG
  GGGTGAAGGCTGCATGGAGCCCAACCTTGCTCCTGGCCTTCCTGTGCTCAGGCCTCTCCCCTTTTCGCC
  AGGGCTTCCTGGTGCCTCTCCCATGAAGAGGGAACTTCCCCTCCACTCCCATGGAAGACCCAGGTTTGG
  GCTGTTGCCAGCTTTGAGGCCCCCTGTGGGCTTGCCTGTCCCAACCCAAGAGGCAGGGCTCAAAGTGCC
  ACCCGGGGGTTGCAAGGACAGCAGAGGACCACCTGCCCTCTGCAGGAAGCTGTCCAGGAAGCCGTGTCC
  TGGGCTGTGGTCCTTGCTCCCCATGGGGAGAGCTGGGTCACTTGGCCTCTTCTAACTGGCCCCAAGCAC
  GCCAATTCTGGAGCATGGTTACTAAGTGGCTCTGAAGCTTCAGGGTCACCACAGGCTTGTCCTGACTGC
  AAACTCATGGAGTGGTTGCAGCCCTGGCTCAGTGTCCTGATGACTAGGTGTGGGTACTAGGCTCCTGCC
  CCTGGTGACACACGTATGGAGTGGGGGAGGGTGGGTGGCTGCACCCCTGGTTCTGGAGTCCCCACAGCT
  GATGGCGGTGTGAGCCTGGCATGCACGGCTGCGCTGGACTCCAGGCTGTTGGGGGAGGTGCCTTTATTG
  CCCAAGCCCACCCCTCACTTGGCCTTGCCCTGGGCAGCCACAGCCTCCATGGCCTTCCGCACCGTTTCC
  TCATCACCCAGGAACTGCATGGGCTTGGTGGGCTTCAGCTCCTTGTTCAGCTCATACACAATGGGGAT
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001014436.3
• Summary: Adapter protein that binds F-actin and DNM1, and thereby plays a role in receptor-mediated endocytosis. Plays a role in the reorganization of the actin cytoskeleton, formation of cell projections, such as neurites, in neuron morphogenesis and synapse formation via its interaction with WASL and COBL. Does not bind G-actin and promote actin polymerization by itself. Required for the formation of organized podosome rosettes (By similarity). May act as a common effector of antigen receptor-signaling pathways in leukocytes. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes.[UniProtKB/Swiss-Prot Function]
• Locus ID: 28988
• MW: 72.1