VASP (NM_003370) Human 3' UTR Clone

CAT#: SC210104

3' UTR clone of vasodilator-stimulated phosphoprotein (VASP) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: VASP
• ACCN: NM_003370
• Insert Size: 836 bp
• Sequence Data:
  Insert Sequence

  >SC210104 3’UTR clone of NM_003370
  The sequence shown below is from the reference sequence of NM_003370. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CAGGAGCTGAGGAAGCGGGGTTCTCCCTGACCACAGGGACCCAGAAGACCCGCTTCTCCTTTCCGCACA
  CCCGGCCTGTCACCCTGCTTTCCCTGCCTCTACTTGACTTGGAATTGGCTGAAGACTACACAGGAATGC
  ATCGTTCCCACTCCCCATCCCACTTGGAAAACTCCAAGGGGGTGTGGCTTCCCTGCTCACACCCACACT
  GGCTGCTGATTGGCTGGGGAGGCCCCCGCCCTTTTCTCCCTTTGGTCCTTCCCCTCTGCCATCCCCTTG
  GGGCCGGTCCCTCTGCTGGGGATGCACCAATGAACCCCACAGGAAGGGGGAAGGAAGGAGGGAATTTCA
  CATTCCCTTGTTCTAGATTCACTTTAACGCTTAATGCCTTCAAAGTTTTGGTTTTTTTAAGAAAAAAAA
  ATATATATATATTTGGGTTTTGGGGGAAAAGGGAAATTTTTTTTTCTCTTTGGTTTTGATAAAATGGGA
  TGTGGGAGTTTTTAAATGCTATAGCCCTGGGCTTGCCCCATTTGGGGCAGCTATTTAAGGGGAGGGGAT
  GTCTCACCGGGCTGGGGGTGAGATATCCCCCCACCCCAGGGACTCCCCTTCCCTCTGGCTCCTTCCCCT
  TTTCTATGAGGAAATAAGATGCTGTAACTTTTTGGAACCTCAGTTTTTTGATTTTTTATTTGGGTAGGT
  TTTGGGGTCCAGGCCATTTTTTTTACCCCTTGGAGGAAATAAGATGAGGGAGAAAGGAGAAGGGGAGGA
  AACTTCTCCCCTCCCACCTTCACCTTTAGCTTCTTGAAAATGGGCCCCTGCAGAATAAATCTGCCAGTT
  TTTATAAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003370.4
• Summary: Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq, Jul 2008]
• Locus ID: 7408
• MW: 31.1