CNOT4 (NM_001008225) Human 3' UTR Clone

CAT#: SC209822

3' UTR clone of CCR4-NOT transcription complex subunit 4 (CNOT4) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CNOT4
• Synonyms: CLONE243; NOT4; NOT4H
• ACCN: NM_001008225
• Insert Size: 1754 bp
• Sequence Data:
  Insert Sequence

  >SC209822 3’UTR clone of NM_001008225
  The sequence shown below is from the reference sequence of NM_001008225. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CCTACAAGTCTCCACACTACTGTGGCCTGACAACAGAACTGAGAGGAGAGGATTAGACTCTGGGGTGCT
  TGCATGGGCAACTGGATTTTTGCATGATTCCTTTATGATTTTGCTTTTAATGTATACACCCAGAAGAGC
  CAATATAAACGTTCCTCATGCCTACAGCTAGCGTGTTACCTCATAGTTGTTGAAGTATTTCTTCATTAG
  GCCTTTAACATAATTTATTAGTGTAATAATTTTGGCATTGTTTCTCATGAGTGATACTATTTCTAACTC
  ATAAAAATAAACTTGTAGTACTAAGATCCCAGGTGAGATTAGCTAGAATTGTTGTTATTTTGGCTCTAT
  TATTGAAAAATTATACAAAGGTAATGGTAGTATCTTGTTTTCTGGTTTGTGAAAAAATAATATCCTAAT
  CTCCTGAAAAATTTGCAAATTAACAAGATAATGTCTATTGCATTCTTTGAATACTTGAGAAGAAAGAAT
  ACATATAAAACAAAATTGGTGGTGTCATTTAATGCTCAGAAGGATATTGTTGGCGATTGTTTTTAGTTG
  TCTAGATTTATATCCTATAATATGCATTACATGTGTAGAAACTGTTTTGTTCTCTCTCTTTTGCACTTT
  TGCACTTTCTTTTTGAATGGTTGTTAATTATGCATAGATGGTTAAAAGATAATTATTGTAGCCTAAATG
  CAATATAATCTCTTTTCTAATGCACTGCCTAGTATACCAGGAAATGCCTCAAAAACAGAACTTCAGTTT
  ATAGTTGACTTATCTGAAACTTGAATACGGAGTAAACATTGTATGTGAAAAGCAACCATAGTAGTGTAA
  CATTGTTACTGAACAAACAGTAATTCTGTGGGATATTGTTTTGGAAATTTCATTGTTTAATAATTAAAA
  TGTGATAATAAGATAATATTATAAGTACCTGACCAGAATGAGACTAAGGCAGAAGAGAAGAGATTGGAT
  GTCCTCTAAAATTAGGCCACCTAAATTGTAACAGATAATTTTAATCAAGAGAATAGTGGAGTACTTTAT
  TAGGACATAATGTGAAGTGATATTTCTGAAAACACATTTCTTGAAATAGTTTTAAAACTGATAATAAAG
  GAAAAGAAACTGTACTTAGTTTGGAGCAGTGGACAAAAGCTGATTCAGAAAACATGACCATATTCTGCC
  TACATGAAGCATCTGGGAAGAAAGGGAAAGTCACACCTCCTCTTGAGATTTTAACCATTATGATAAAAG
  GTATGCTTTAATTTGTTAAAAAAAAAAGTATGAAAGGGGATAATGTGAATACAAAAAAGAAGCCTAGAG
  ATTGAAAATAATAATAATAGAACATGTTATTAGGAGATGGTACTTAATGTCATGTTGGAGTTGGAGTTT
  TCAAGGTTACCTCTATATAAAATTGGATTATTTTCTGTTAGATATAATGTGACAAATTAAATGACTTTA
  TCCCAAGGTACAGTGAGGTAAACAAAAATCTATTCTTAGATATCCTTAGATTTGGTTAACTGAGGCTGT
  AGAAGCAGCGAATTGTCTAGCCATTTAAGTTATTTTTGTAAAATTCAGCATAAACATCAATCTCTAATT
  TCTATCTAGAAGTGACTTTAAGACATTACAAGAACATTGCCAATTTGGAAAATGCCAATAAAATACTGT
  AGACTTATTAATATTGTAGACTTATTATCAGTCTTTTACTATAGCTTAATTTAATTATGGTTTTCATTT
  TTACTGTAAGAGGCCAAGACTTCACTTAT
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001008225.3
• Summary: The protein encoded by this gene is a subunit of the CCR4-NOT complex, a global transcriptional regulator. The encoded protein interacts with CNOT1 and has E3 ubiquitin ligase activity. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2010]
• Locus ID: 4850
• MW: 68.6