Acetyl CoA synthetase (ACSS2) (NM_001076552) Human 3' UTR Clone

CAT#: SC209651

3' UTR clone of acyl-CoA synthetase short-chain family member 2 (ACSS2) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: Acetyl CoA synthetase
• Synonyms: ACAS2; ACECS; AceCS1; ACS; ACSA; dJ1161H23.1
• ACCN: NM_001076552
• Insert Size: 775 bp
• Sequence Data:
  Insert Sequence

  >SC209651 3’UTR clone of NM_001076552
  The sequence shown below is from the reference sequence of NM_001076552. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  TTCAGCCACCGCTGCCTGACCATCCAGTGAACATGATCCTGACCTTTACCTAGGATTCCTCCTGCTCCA
  AACTTTGCCCATCCTCTTTGCCCCCTCAGGAGTGCTGAGGGCCAGTGTTGACCCACACTACCCTCCCTT
  GACCAGCTGTCTGGGACCGGAAACCAGCTTTGTCTCCAGGTAGAGACAACATCCTGTGACTGCCAGGCA
  GAAAGGACAGGGCCCAGGTCAGCCTCAGTCTGCTGTGCCTCCAGACTGCAGAGCTCTCAGAACCCAGAA
  CAGAGACGAAAAGGCTACCTCTCCTACCCAAGTTAAGTGTTCAAAGGGGATGTGAGGGCCTCCACTGAA
  GCAGGGAGGCAGCTGTGTAATCCTATGTCAGCTCTCTTAGGAAGCCCCAGTACTTATATTGGGCATGCA
  CTTGCCCTTAAAAACAATGATTTGTGAGTCCAGGAACAATTTACTATTTTTAAAATATTTTGCTGCTTC
  TGTTCTGGGTCTGAATTCCCTTTTGTGCCAGATGCCAGTACTGTCTGCCCATTGGCTCCAGGGGCTGTA
  TGGGCAGATTCAGTCTCCAGAGGGTATTCAGATCATCTGCTTCTTTGAAGGAGTAAATGTGTTTTGTTC
  CTAGGGCCAGAGGAGCTTGTCTTCCTTGTCCTCTGTTCCCACCCTCCCCTGAACAGAACCCAGCCCATA
  AGAGACATTCTCAGATGAAACTCTGTTTTCTTGCCCCAGTCAGGCTCAAGCCCTGTGGTTGTAGGAATA
  AAGCCTGTGATCTCAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001076552.3
• Summary: This gene encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009]
• Locus ID: 55902
• MW: 28