UGT8 (NM_003360) Human 3' UTR Clone

CAT#: SC209628

3' UTR clone of UDP glycosyltransferase 8 (UGT8) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: UGT8
• Synonyms: CGT; UGT4
• ACCN: NM_003360
• Insert Size: 1967 bp
• Sequence Data:
  Insert Sequence

  >SC209628 3’UTR clone of NM_003360
  The sequence shown below is from the reference sequence of NM_003360. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CATATTAAACATGAAAAGAAAGTGAAATGAGCCAACAGCCCAGGTGATAGAAATAAATTGGTTCACTCA
  TTGAATTTTTATTGCTATTATTTAGTCTAACAGCTACTAAAAGTAAAACATCAGTAAACAATTCTAACA
  TGCCCTTATGAGATCTACTAATGAAATTCTGTGGAATTAAGATGGCTGTAAAAAGCACAAACCTAAAAT
  GCAGAAATGTATTTTATTCAAATACTGATGTAGAGAGTTTTGGCACTGAACCTTTTAGAAGCCTTAATT
  ATTTAAATCAATTCAGTGACTGTGTCAGACCTTAGTTTTAAATCTTGATATGTGCGTGTCCCGGATCAG
  GAATGGTTTCATTTTTCTTAATAATGTGTGTGTGTGTATGTGTGTGTGTGTGTGTGTGTGTTTGTGTGT
  GTGTGTGTGTGTCCTAATTAAGAGAATTTTTACTGGCTGCTTGTTACATTTGTTGAGGGTACAACACAC
  TCAAGAATAAAGTAAAAGGATAGTCAGGATCCAGATGTTTCTTTTCTAGCATTTAATGTGTGTGAACTC
  AGAACACTACCATGAAAGAACACTTCCCCCCAAAACTGGATGCAGAGGAAGAAAAAAAAAAAAGAAATG
  GCACAGTTTTTTGTATTTTTTTTTTTAGTTATTTTTTGTTGTTGTTGTTTGTATTGTTTTTCTTTTCTG
  ATAGAGTATGGCCACTTCTGGGGGAAAAACATGTAATTAAGTAAATGTATACATTGGTTTTCACGTTTT
  ACTTTTGTTTTCCCACTACCAATAATTTTCCTCTGGAAGAATTTTATAGTTTTTTCTATTTCATTTTAA
  TGAGTAACAATATGTTAAATGCATAATTAAGACAAAGCAATGAAATTCTGACTTTATTCAAAGTACTGA
  AGATTATTGCTTCTAGGGCATTTTTAAACAGCACCATTGTATTGTTGAATGTTTATGTAACTGATGGCT
  TTTCTATAATGTAATTTTTGAATGTTCAGGTGTTACATTTCCAAAGTTTAACTTTTAAAAAACCATCTT
  CTGATCCCTTTTATTGTCTGGGCCATACAATCTATATTACATAGGTGCCAACATTTAATTCTTTTAAAT
  GGAACATTTGCAGTTTTCCATATTGGTACCTGCTTTTTCTGGAGAGGTTTGAGATCTTGTTAACAAATC
  AGACTTTACACTATATACAGATGTGACAGATAAATTGAACCACTTGTTTGTGAAAATGAGTTCCTGTCA
  TCTTCCTCATTCACTCAGCCCTCACCCCAGCAGCCTTTTCCGGTCATTGTAGCTGACATGGAGCAGTGA
  CAGTATTCATTTGAGAACAGGGATGCAAGTCACAGACATCATAAAATCAGGGTCTCCCTGCTGAAGCAT
  TCACTAGTTGGCAACTATGAATTTATTCCATGTCATTCTGTTTACTTAGCACTTGCACTACCCTTGTTG
  GTTGAGTGTATGCTTTATTTGTTTCTAGTTTGAAATCCCACATCTGATAGCTGAGAGTAGGCAAATACA
  ACATTTACCTAATGTCATTCACTAACATGGAAGAGTTGTGAAAATTCTAGAGTGCTGTAAATCCTTGGC
  ATACACTATGACAAACAACTTCATTACTCTCCCACCAGGAGCTGCTCTCCTGCACTTAGAAATAATGTC
  ACAAGTAGTTTTCTAATGTACAATGCAGACAAATGTACTGCTCTCTGAATACTTGAAGAAATGGTATTA
  TACATACATAGAAACTTATTAGTTATACCTTTTCACAATCTTATTACGATGTTGCCGTTAAAAGGGAAA
  AAAGACACAGGCAATGAATGGTGGGATAGTAAGAGGACTTAGAGTGTATGAATGAGTTGATTTTACTTT
  TTTGGAATTTGATTAAGTTGACAGTAGGCACTGATTGGATGATTAAACATAAGTTAATCTCCACTGTGA
  TAAAAACTTAAATAATAAACATGATTTAAAATAGA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003360.5
• Summary: The protein encoded by this gene belongs to the UDP-glycosyltransferase family. It catalyzes the transfer of galactose to ceramide, a key enzymatic step in the biosynthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central and peripheral nervous systems. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2011]
• Locus ID: 7368
• MW: 77