RAB30 (NM_014488) Human 3' UTR Clone

CAT#: SC209501

3' UTR clone of RAB30 member RAS oncogene family (RAB30) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RAB30
• ACCN: NM_014488
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC209501 3’UTR clone of NM_014488
  The sequence shown below is from the reference sequence of NM_014488. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AGCTATTTGACTTGTTGTAATTTCAACTAAAGGCTGAGGCACGGAGAAGAAAAGGAATCAGCAACTGCC
  CTGATGGGCCATGAGATGCTGGGGAGATCTGGCGATGACTGTGGCTCCCGCTCTCTGACCTTCTGACTC
  CTGTGGGCTCCTGAGCTTACAAAGCATGGCAGGCCAAGGGCCTCGACCACAGGCCAGCATTAGCAGAAC
  ATATAATGGTTTCACCCTTTTCCAGTCTGGAGTTGGAGCAAGGAGAAAATTGCACTAGGCACTCCATGA
  TCTGCAGAGCATGTTGCTTTTGTTTTTTTAAAAAGCAAGTAAAAGCGCATTCCTAAACAGAGTCCGGGG
  GGAAATGTACTGTAGGGATCCCTCCTGCACATCAGTGGGTGCATGTGGGAACTCTTCTTTAGGGCTTCG
  GTGGTAATCATCTGGGGGCCCTGGTTTTCTTGTCTACCAGATAAACCAAAACTGGGAAGGCCAAGTACA
  GTCTCTGTGGTGCGTATTGACGACCCCATGGAGATTTTGAAAAGTGTTATTTCTCTTGTCCACAGGCAC
  TTTCAAGAACTTTGGGATGTAAAAATGAAATGAAACCTTTACCCATGACCAACAGTAACAATCATTGTT
  TTAATAATATATACAAGCTCCATAACTCCTTTTGGTGCTAATGATTCCGCTATTTCACAGACCAAACGT
  TTTAGTACATTAATGTCCTTAAATGTGTTATATAGAAATAAAAAAATGGGGGAAGTCCATGAATCAGCA
  CTCTTTCTCAAAGTCTCATTACTACCTTTTTAAAGGTGGATTTTTGGGAAAAGAGAATACTTTTCCTTT
  TATGTTCCCCTCTGAATCTCTACCACTTTCTTGTCTCTCCAGCCCTTTATGTAAATGAAAACGTTTGCA
  AAATGAAAAAAATAAACAAATGGTTGATGACATTCTAATATAAAAACTTAGCTCTAACACATTTTGAGT
  AGTATGAGGAAACAGACCAGATATGACCAAAATACTGTGGATTAATGGACTGTAGTGCAGGGCTCCTAG
  CAGAGCACAGTCCTTGATAGTAGGTCACATCTTTTACTTCCTGGTACTCTCCATCTTGGACAGACCAAG
  GCTTAGGATTTTGTAGATTTTTTAGTATGATAAGTGAGAATAGCAACAGATGAAAAGTCTCACGACCCA
  TTTTTATATCTTCGATGATAACCTTAGAAAAATTCTGCTCGTGGAAGCAGATTGAACTGTCATTTATCC
  CCTCCTTCTTGTAATAATGTTACCTTCAGACGCTTAACCATATGTTCTTTCTTTTTTCTTTCTCTTTTC
  CTCTCTATTTTTCTGGTCTCTCCTTTTCTTCTTCCCTCCCTCCCTCCCTCCTTCCCTCCCATCCTTATA
  TGCCTGTGTTACTGCAAATCTCCAATTCAAATGGAGGTGTATATGCCTTTACTTAGCCAATTTAAAAAC
  AGGACTCAAAATGGGAGATGTGCTGCTGAATATGTGTTTCTTTTTCTTCAGTGCCATACTTTGATAGCT
  TCCAATTTGTTCACTGATATAAATGCAAATTGGGAAAATAATTAAACCTGTATGCTGTTGATGCTGTCA
  GATGTTTCTGTAGCTTGCTGCTTAGCATAACTGAGGGATGAGTAATGGAATAAGGATATGATGTGTAAT
  TCCACTGTTGCTGTAGATGGTGACAATCTCAGTTCTGATATGTTGATGTTTGAAAGCTATTTAATTGTA
  AAGAAATTAGTCATGTAGAGACATGATGTGGATAAAGCATTTCTGGCATTTCTCTAGCAGGTTCTAGAT
  TGACCAGTGTCTTGCACATGAGATTATGCATATTTTTTAAGGGGCTGATACTTTTGGTTGGCCTCACTG
  TGTTTTTCCTGATCCTGTGGGAAAGAGAAAGGCATCCCAGAAGTTTGACTTGTTTTTCTTTTAGAATCT
  GTCTCTGCAGGAAAGAAAAGTCATTTTAATAAAAACCAAAATAATTTATAATAATTGTTGCCTTTCCC
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_014488.5
• Summary: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity). Required for maintaining the structural integrity of the Golgi apparatus, possibly by mediating interactions with cytoplasmic scaffolding proteins.[UniProtKB/Swiss-Prot Function]
• Locus ID: 27314
• MW: 76