Tyrosyl tRNA synthetase (YARS) (NM_003680) Human 3' UTR Clone

CAT#: SC209458

3' UTR clone of tyrosyl-tRNA synthetase (YARS) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: YARS1
• Synonyms: CMTDIC; TYRRS; YARS; YRS; YTS
• ACCN: NM_003680
• Insert Size: 772 bp
• Sequence Data:
  Insert Sequence

  >SC209458 3’UTR clone of NM_003680
  The sequence shown below is from the reference sequence of NM_003680. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AAATCGCTGAAAGGGGGGAACATTAGCTAGCCAGCCCAGCATCTTCCCCCCTTCTTCCACCACTGAGTC
  ATCTGCTGTCTCTTCAGTCTGCTCCATCCATCACCCATTTACCCATCTCTCAGGACACGGAAGCAGCGG
  GTTTGGACTCTTTATTCGGTGCAGAACTCGGCAAGGGGCAGCTTACCCTCCCCAGAACCCAGGATCATC
  CTGTCTGGCTGCAGTGAGAGACCAACCCCTAACAAGGGCTGGGCCACAGCAGGGAGTCCAGCCCTACCT
  TCTTCCCTTGGCAGCTGGAGAAATCTGGTTTCAATATAACTCATTTAAAAATTTATGCCACAGTCCTTA
  TAATTGGAAAAATACTGGTGCCCAGGTTTTCTTGGAGTTATCCAAGCAGCTGCGCCCCTAGCTGGGATC
  TGGTACCTGGACTAGGCTAATTACAGCTTCTCCCCAACAGGAAACTGTGGGATTTGAAAAGGAAAGGGA
  AGGGAAAACAGAGAACCTAGTGGTCTACCAAGTGGTTGGCAACTTTCCCAATGTCTGCTTACTCTGAGG
  CTTGGCACTGGGGGCCAGGGCCTGCCCCAGGGCTCCTGGAATTTCCCTTGATCCAGCTAGGCTGGGACA
  CTCCCTAAATCAGCTGCGTGTTGTTAGCATCAGGCAGAATGAATGGCAGAGAGTGATTCTGTCTTCATA
  GAGGGTGGGGTACTTCTCCATAAGGCATCTCAGTCAAATCCCCATCACTGTCATAAATTCAAATAAAAT
  GTCTGAACAAGGG
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003680.4
• Summary: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine. [provided by RefSeq, Jul 2008]
• Locus ID: 8565
• MW: 28