KCNN1 (NM_002248) Human 3' UTR Clone

CAT#: SC209209

3' UTR clone of potassium intermediate/small conductance calcium-activated channel subfamily N member 1 (KCNN1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: KCNN1
• Synonyms: hSK1; KCa2.1; SK1; SKCA1
• ACCN: NM_002248
• Insert Size: 1709 bp
• Sequence Data:
  Insert Sequence

  >SC209209 3’UTR clone of NM_002248
  The sequence shown below is from the reference sequence of NM_002248. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  ACGCCCGTGGCCCCCTCGGACTGCGGGTGACGGCCCTGCCCGCCACCAGACCCCTAAATCTTGGCCATC
  GTGTGGCCGCCACCTCCGGGAAGCCTTGTACAGTGGCGCCTCTTGGAGTTCAAGAAGCCAACGCTGAGT
  CAGGCTGAGTGGACTGAGGCCTGCCCCGCCCAGACTGCCCAGGCAGAGGGCAGGGCTGGACCATGGGTG
  AGGGCAGGGGAGCCCGGAGCTTCCTCTGGTCACCTGGTCCCCCGACTCTCCCCAGGCCCCCGGTGGGCA
  TGGAGCAGCCCGGGGAGGGGTCCGTGCTGGTTCTGAATAAAGCAGGACCCGCCTAGTGGCTGCCTGTGT
  GCATGGCTGGAAGGCACTGGTGATGTCCCAGGAGGTAGACCTCCAGCCCTGGGTACCAAGATGAATGTG
  GGAATCAGAAAAACCTGTTCCCATCACCGGCCTAGCCTAGAATCCTAGCCTAGAAGCCCTCTCTCCCTC
  TGGGCTGGAGCTCAGTGAGGGACAACTCTCTAGGGACACCTGTACCAGCCCCACCTGGCGCTGAGATCC
  CTCAGACAGCATGGCCCAGCCCTGGCCAGAAGCATCGCTCCCCTTCAACCAACCGCGTTGATGGACACC
  CACTGTGTGCCAGGCCCCAGCGGGGCCCATGGGGGAGGTGACCTGGGTGAGGAAGGTCATTTGGGTTTT
  TGTGAGATTTGTATTGAGCACCTGCTGTCTACAGAGAATGTGATGATGTCAATTACCACACTGATTCCT
  CATCAGAACTTGACCATGGTGGGATGGGGGGGAAACTGAGGCCCAGAGAGGTGAATCTACCTACCTGGG
  ACCACACTGCGAGGAAGGATGGTGCATTCAAACTGGAAATCCCCTAGGCCAAAGTAAAGAACCGGACTG
  GCTGGGCACAGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGGGTAAAGCGGGCGGATCACCTGAGG
  TCAGGAGTTCAAGACCAGCCTGGGCAACATGGTGAAACCCTGTCTCTACTAAAAATATAAAAATTAGCT
  GGGTGTGGTGGTGCACGCCTGTAATCCCAGCTACTTGGGAGGCTGAGGCAGGATAATCGCTTGAACCCA
  GGAGGTGGAGGTTGCAGTGAGAAAAAACCTGGACTTGGTGGAAGGGACTCCATGGAGGAGCCATGGGGA
  CGTCAGGAGGAGGGGTTATTTGGTGACATCCAGGGGTCAGAGAGACCCTTCAGGGGTGGGCTACAGGGG
  AGGGTTCCAGACACAAGTAGAATCAGAAAGGTCCCTGGAGGCCATGGGCTTGGGTGCTGGGAGCAACAG
  GCATTTACTTGGCAGATGCTGAGCCCTGGGTGGGGCGAGGAGGCCTGGCTCCTGGGGAGACGACGCTGT
  GCATAGCCGAGGAGCCCCAGGTCCTGGCAGCCTCTGATAAGGCCATCCATCGCCTGGCTCAACGAGATA
  ACTAGGCCGTGGCTGGTGCATGAATGACCAGCTTGGGCCCACGCACGAGAAGGGTATGAGGAGGTGCTG
  GTCAGACCCGGGTTCGAGTCCTGACTCTGCCACTGCTGGGTGACTCTGAGCAGTGGCTGCCCCTCTGGG
  GCCTTGGTTGTTTCATCTGTAAAATGGGGTGACAGTCTATTTAACCAGAACTCCCTAAAAAGGGCCAGG
  CAACTGGTAGGTGCTCAATAAATGCTCCTTCCCGCCTGAGGGATCACACTGGA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_002248.5
• Summary: Action potentials in vertebrate neurons are followed by an afterhyperpolarization (AHP) that may persist for several seconds and may have profound consequences for the firing pattern of the neuron. Each component of the AHP is kinetically distinct and is mediated by different calcium-activated potassium channels. The protein encoded by this gene is activated before membrane hyperpolarization and is thought to regulate neuronal excitability by contributing to the slow component of synaptic AHP. The encoded protein is an integral membrane protein that forms a voltage-independent calcium-activated channel with three other calmodulin-binding subunits. This gene is a member of the KCNN family of potassium channel genes. [provided by RefSeq, Jul 2008]
• Locus ID: 3780
• MW: 61.8