WAVE 1 (WASF1) (NM_001024936) Human 3' UTR Clone

CAT#: SC209197

3' UTR clone of WAS protein family member 1 (WASF1) transcript variant 4 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: WASF1
• Synonyms: NEDALVS; SCAR1; WAVE; WAVE1
• ACCN: NM_001024936
• Insert Size: 733 bp
• Sequence Data:
  Insert Sequence

  >SC209197 3’UTR clone of NM_001024936
  The sequence shown below is from the reference sequence of NM_001024936. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GAATTTGATGAAGTAGATTGGTTGGAGTAAGAAAAATGCATTGATAAATATTACAAAACTGAATGCAAA
  TGTCCTTTGTGGTGCTTGTTCCTTGAAAATGTTTGGTCATTCTAGTGTTTTGCTTTCTTTTCCTTATAA
  TAAATGACCCTTTTCCTCCATAACTTTTGATTTCTAAGGAAAATATTAGCATACATTTCAAACTAAATG
  TTTTACAGTGGCTTATCTTTTTTTTCCCCCTGAAAAGACTAATTTGGTCAAATAAACCACTAAGTATTA
  AGCATGGACAGCTGTTGTTAGAGTAGCAGATTCAGTTTTTTGATATATCTTAATTGTGTACTTTGTGAA
  TTTTAATTTAAAGAAAGCAACTGAAATTGAAATCTTGAGGGCAGCTGTGTCTACTAATGAGCCTTATTC
  CATTTCCTGATGTTTTAAAAGAAGAAACACTGCCTTGATTATACGAATACACTCAGAAAGTACATTTAG
  CTTGTAGTGTTGAATTCTCTTAAAGGAATGCTTGAATTTTTTCATTATTGTTTTATTGTTTTTATATAC
  TTGCCTTATTTGAATGTTTAGCAGTATCCCCTTCCCACTTATATATTGTGTGATATGATTTTGCTTGCC
  TATAGGAGTTAAAAACTTTTCCATGTGAAATACTCTGACTTAAACATACATGTAACTTACATAACTGTT
  AAGAATAACAGTCTGATTTAATAAATGGTTCATTTTAAAAGTT
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001024936.2
• Summary: The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
• Locus ID: 8936
• MW: 29.3