TRAF6 (NM_004620) Human 3' UTR Clone

CAT#: SC209041

3' UTR clone of TNF receptor-associated factor 6 (TRAF6) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: TRAF6
• Synonyms: MGC:3310; RNF85
• ACCN: NM_004620
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC209041 3’UTR clone of NM_004620
  The sequence shown below is from the reference sequence of NM_004620. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CAGCCACGAAGTACTGATGCAGGGGTATAGCTTGCCCTCACTTGCTCAAAAACAACTACCTGGAGAAAA
  CAGTGCCTTTCCTTGCCCTGTTCTCAATAACATGCAAACAAACAAGCCACGGGAAATATGTAATATCTA
  CTAGTGAGTGTTGTTAGAGAGGTCACTTACTATTTCTTCCTGTTACAAATGATCTGAGGCAGTTTTTTC
  CTGGGAATCCACACGTTCCATGCTTTTTCAGAAATGTTAGGCCTGAAGTGCCTGTGGCATGTTGCAGCA
  GCTATTTTGCCAGTTAGTATACCTCTTTGTTGTACTTTCTTGGGCTTTTGCTCTGGTGTATTTTATTGT
  CAGAAAGTCCAGACTCAAGAGTACTAAACTTTTAATAATAATGGATTTTCCTTAAAACTTCAGTCTTTT
  TGTAGTATTATATGTAATATATTAAAAGTGAAAATCACTACCGCCTTGTGCTAGTGCCCTCGAGAAGAG
  TTATTGCTCTAGAAAGTTGAGTTCTCATTTTTTTAACCTGTTATAGATTTCAGAGGATTTGAACCATAA
  TCCTTGGAAAACTTAAGTTCTCATTCACCCCAGTTTTTCCTCCAGGTTGTTACTAAGGATATTCAGGGA
  TGAGTTTAAACCCTAAATATAACCTTAATTATTTAGTGTAAACATGTCTGTTGAATAATACTTGTTTAA
  GTGTTCCTTCTGCCTTGCTTACTTATTTCCTTGAGGTTACGAAGTAGCATCTTCCCCAGAGTTTATAAT
  GCTGAGAACCACGTGGATACCAACTGCTCATTGTTATGCTATGTAACCCTTTTTGTCTATTCAGTGCAG
  AGTGAATTTCACAGCTCTGCATATGTCTTCATTTGTTTAATGCTTACAAGACAGGAGATGCACACATAC
  AATCAGCAACATAAAAATTAAAAGTGACCCAAGTAGTCAGCGCATGTGGCATCTCATTGGTGGTGACAG
  AAGCTATGTGAGCCAGAAGTTTTCAGCTCTTTTGAATACCCTCTGGTTTATTTCGATTAAAAAGAACAA
  AATTGATTTCCTAAAATCAGAATTTTTTAAAACTTGGGAGATGATTGGAGATACCTAGGAGGTCACCAA
  ACTAGGATTAGAAGTCACAGTGGTTGTATCACAACTTAGCTTGAGTATGTTGCTGTAGCCTAACAACTG
  CAGGTTCTGAGAAGGATCCTGTAGAATCCTGGAAGTAACCAGATTTTCCTAATAGGGAGATGATTTTTT
  TGTGTGCCATCATGTATTTGTTAAAGGCCTATATATAGATATAAAATATCGTGGAATCTAGTTCTCAGG
  GAGACCCGCAACTAGTATAAGCTTATAAAGGATCTAAAGATCCATCCACCATTTAAAGTTGTCTGGTAA
  TGAGAGATGACATTGTATCCCCCAGAGAGGCCAAATCAGAGTCGCCAGCCAGCGTTCTAGATCAGCCTT
  AATTTCAAGAGAAAGCCAAGGACCTCATCTGCAGGGGAGTGTGGTTTTCAGCCCCAGCGAGTGTCACTT
  TGAACTTTCCCTTTGCTTTTTTCTCTCTTCTCCCTCCCCACCCACCCTTAGGCTCCTGATCTGGTGAGT
  TTGTTATGGAGTGAAAATAAAAGTCAAGCAGAGACCTTGTTTCCCGTGCCACCATTAGTACCACAAGCT
  CATGGCTAGTTACCACATTACTTCCTGGCAGTTTGTGTCCCTCAGCTGTGCCTTCCAACCAGCGCCTGA
  GAATCACTGCATACCACCCTCTAGGTAGGGAAACCTACACTGCTGCTGTTCCTGTGATTATTTTACAAT
  GAATAAATAATTGTCAAGTTCCATTTAAAAACTGAACAGTAGTATTTTTGTATTTGCGTAGAAAAAGCC
  TGAAGGAAATATACTAAACTTTTTGTTGGCTTATTTTCCTTTGCGCTTGCTTATATTTTTTACATTTTC
  TACAATAAATGTGTACTTTTATCGGAGAAAAAAATTAAATGTTGCCACAAAACATTTAATCTCCACGC
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004620.4
• Summary: The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins are associated with, and mediate signal transduction from, members of the TNF receptor superfamily. This protein mediates signaling from members of the TNF receptor superfamily as well as the Toll/IL-1 family. Signals from receptors such as CD40, TNFSF11/RANCE and IL-1 have been shown to be mediated by this protein. This protein also interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways. This protein functions as a signal transducer in the NF-kappaB pathway that activates IkappaB kinase (IKK) in response to proinflammatory cytokines. The interaction of this protein with UBE2N/UBC13, and UBE2V1/UEV1A, which are ubiquitin conjugating enzymes catalyzing the formation of polyubiquitin chains, has been found to be required for IKK activation by this protein. This protein also interacts with the transforming growth factor (TGF) beta receptor complex and is required for Smad-independent activation of the JNK and p38 kinases. This protein has an amino terminal RING domain which is followed by four zinc-finger motifs, a central coiled-coil region and a highly conserved carboxyl terminal domain, known as the TRAF-C domain. Two alternatively spliced transcript variants, encoding an identical protein, have been reported. [provided by RefSeq, Feb 2012]
• Locus ID: 7189
• MW: 76.9