PKIG (NM_181805) Human 3' UTR Clone

CAT#: SC209036

3' UTR clone of protein kinase (cAMP-dependent catalytic) inhibitor gamma (PKIG) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PKIG
• Synonyms: PKI-gamma
• ACCN: NM_181805
• Insert Size: 703 bp
• Sequence Data:
  Insert Sequence

  >SC209036 3’UTR clone of NM_181805
  The sequence shown below is from the reference sequence of NM_181805. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CAGAGCAGCGATGGGACCACCTCGTCTTGAATCTGACCTTGTCCAAGAAGGCTGGACGAGAGACCTTCT
  GTCCCCTCCCAGAGGGGGAACCCTGGCACTGGCCCAGCAGCCTCTTCTCTGAGCTCCATGTCCCAGATA
  AACCAGGCCAGACTGAGAAGGCTCCCCAGAGGCCTCTGTGGCCTCCACTCCGGGAAAGCCCTCTGCCCA
  CACCCACAGGCTTCACATTCCCACCACCTTCGCACCGTGCCCAGGTACACTTTCAAGACACTGTAACCA
  CAAGATGTTATTTATTGAGCTGGCGCCGGGACTTGGGCGGGGCCTGCCCTACAGTGAGCAGCCCACACA
  GGAACGCTCCTCTCGCGAGCGGCCCGGGCAGGGACCCTGTCCCAACACCAACACCTCCTCTCCAGCCCA
  ATCTTCTGGGTCCAGACCTGCTTGTCCCTTTTTTAGAAAACACTTTTAAACTTTTTAAAAATTTTAAAC
  CTTTTTTCAGCAGATATGGAGAGAGCTGACAATCAATTCACATTTTTTAAGCCATTTTAGCTAAACTGT
  CATTGTGCATCTCTGAGGTTCCCTCATGGAGCTCCACAGATCCATTTTTAGGGAAGGGATTTTGGCTCA
  AAACGATCTGACCACCTCTGCCCTGTCCACCAGGATAAGTGACACCTAGGACCCAGGAAATAAATGCCG
  ATGATTTGTGTGA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_181805.3
• Summary: This gene encodes a member of the protein kinase inhibitor family. Studies of a similar protein in mice suggest that this protein acts as a potent competitive cAMP-dependent protein kinase inhibitor, and is a predominant form of inhibitor in various tissues. The encoded protein may be involved in osteogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
• Locus ID: 11142
• MW: 25.8