ABCB11 (NM_003742) Human 3' UTR Clone

CAT#: SC208868

3' UTR clone of ATP-binding cassette sub-family B (MDR/TAP) member 11 (ABCB11) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ABCB11
• Synonyms: ABC16; BRIC2; BSEP; PFIC-2; PFIC2; PGY4; SPGP
• ACCN: NM_003742
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC208868 3’UTR clone of NM_003742
  The sequence shown below is from the reference sequence of NM_003742. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CTAGTCACCACTGGATCCCCCATCAGTTGACCCAATGCAAGAATCTCAGACACACATGACGCACCAGTT
  ACAGGGGTTGTTTTTAAAGAAAAAAACAATCCCAGCAGGAGGGATTGCTGGGATTGTTTTTTCTTTAAA
  GAAGAATGTTAATATTTTACTTTTACAGTCATTTTCCTACATCGGAATCCAAGCTAATTTCTAATGGCC
  TTCCATAATAATTCTGCTTTAGATGTGTATACAGAAAATGAAAGAAACTAGGGTCCATATGAGGGAAAA
  CCCAATGTCAAGTGGCAGCTCAGCCACCACTCAGTGCTTCTCTGTGCAGGAGCCAGTCCTGATTAATAT
  GTGGGAATTAGTGAGACATCAGGGAGTAAGTGACACTTTGAACTCCTCAAGGGCAGAGAACTGTCTTTC
  ATTTTTGAACCCTCGGTGTACACAGAGGCGGGTCTATAACAGGCAATCAACAAACGTTTCTTGAGCTAG
  ACCAAGGTCAGATTTGAAAAGAACAGAAGGACTGAAGACCAGCTGTGTTTCTTAACTAAATTTGTCTTT
  CAAGTGAAACCAGCTTCCTTCATCTCTAAGGCTAAGGATAGGGAAAGGGTGGATGCTCTCAGGCTGAGG
  GAGGCAGAAAGGGAAAGTATTAGCATGAGCTTTCCAGTTAGGGCTGTTGATTTATGCTTTAACTTCAGA
  GTGAGTGTAGGGGTGGTGATGCTACCATTACTGTGAGGACCTACCAGTGTGGCTGGAGCAGGGACTCTC
  TCCCAGGCCTTTTACTCCTCAGCACCTCCCTGCATACTGATTGTTGTTTTTAGTTTCTGTGAAATTATA
  TTCATGAAATGAAAATAGCGCATTTTACTTTGCTGTAGTTTCATAAGGTTTTATACAAAAAAGCAAGTA
  AATATGGCAGAAAAGCACTCATTTGCCCCTGCTCCCTCAAAACACCACAGAATGACATAGAACTAAAGG
  CGGCAGGAATCTACAAGAATGAAGAAAACACAGTGATGCCACCTGCAAAATCTTGGGAGCCAGAAAGCA
  AATGGACAATTGATAATAGAGTTACAAGATGAGAGAAAACAAAAATGTAACCTGTTAGTTGGGGGAGCC
  TAGAAACATCCTGTTTTGTACCACAGACCCCTAGAAAGTTTCAAGATGTAAAAACACTGGATCCTTCTG
  GAAGGAGGAGACAAGGGGACAGAGGGACTGAAGACAGAAGAATGGACCAAAAGCCTGTATGGAAACAGA
  ATTGCAGAGCCCCGCCTGCAGCAGGATGGCTGCCTTTCCCCAGTTCCAGCGAGAAACTGCATACTCCCT
  CTCTGAGGAGGCTCACAGGGAGGTTTGCACTTAGAGACACAAGATGAAGTTGAAGGGGCAGCTAACATA
  CAAGAGACAGGGGGATTGAATGTAAAGCTGTGTATGAACAGAAGAACTCCCAGCCCCTCTCTTCTGGCA
  GGAGATTGAAGAATGATTTTCTGGGGAAATTGGCCAATCTTAGAAAAGAGGTCGGGCCAGGCGCTGTGG
  CTCACTCCTGTAATCCCAGCACTTTGGGAGGCCAAGGCGGGCGGATCATGAGGTCGAGAGATTGAGACC
  ATCCTGGCTAACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAAAATTAGCCGGGCGTGGTGGCGG
  GCGCCTGTAGTCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATGGCGTGAACCCGGGAGGTGGAGCTTG
  CAGTGAGCCGAGATCGCGCCACTGCACTCCAGCCTGGGCGACAGAGCGAAAAAAAAAAAAAAAGAAAAA
  GAAAAAGAAAAGAGGTCAAGGACTCCGTCCTTGGAATCCTAAGAAAATTTTCCAGCCGTATTACCCTTC
  TATGAAGCCCACCTGTCAACCAACAAGCACCCACTCGATCAGAGCTTCCCCAGGCTTTTTGGTGTCTCC
  TCCTTGCATGGGAATTGACTTCCAAGGACCACCAGACACTGAGGAAGTATTTTAACATATAAAGCAAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_003742.4
• Summary: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is the major canalicular bile salt export pump in man. Mutations in this gene cause a form of progressive familial intrahepatic cholestases which are a group of inherited disorders with severe cholestatic liver disease from early infancy. [provided by RefSeq, Jul 2008]
• Locus ID: 8647
• MW: 75.5