CYP39A1 (NM_016593) Human 3' UTR Clone

CAT#: SC208761

3' UTR clone of cytochrome P450 family 39 subfamily A polypeptide 1 (CYP39A1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CYP39A1
• ACCN: NM_016593
• Insert Size: 816 bp
• Sequence Data:
  Insert Sequence

  >SC208761 3’UTR clone of NM_016593
  The sequence shown below is from the reference sequence of NM_016593. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  TGCCGAATTGAATATAAACAAAGAATATGACATCTGTTGGGCCTCACAAGGACCAGGGCCTTCTGGAGG
  AGTGGCACTACCCCACCTGGCAGCACCTAGACCTGAGCTCTACAAAAACACACTGCTTCACTTTGTTTT
  AGGACTTAGTTCAAGAACACATTCAAATGGTGCATGTGTTTGGTATCTTCAACAGTAGACCAAGAATCT
  AACATCACTCTCAGTAATATAGAGACCGGAATACATGGTTTATAGGAAATGATCAAATGATCCAAAAAA
  ACTCCACATTTTTTAAGAAGTTGGAATTTGATTTCATGCATAACTGTATTAAAACATTAAATAGAAATA
  ATGTCATTTGAATGAAAATCTTATCACATTAAATTCACTGTGAAGGCAGCATACTTAAATTTTTATTTT
  GAAAAGTCTAAAAGGCTTAGATTTTTAAAATTTAATAATTATTTCTACAAATTTTCTATTTTTCTTGAG
  GTGATTCTCAACTAGCAATTGGAACTCCTAGGCTCTATTAACATAATTCTTTATTGTAAACGTATCTAA
  TGCTAAAAGTAATAAAATGGTAGTTTTCTGAGACCTGTGAGGACAGGAATGGTGTCTTACATTCATTTC
  TACACTTTATTATGCTCAGGATTGCACCTTCTTTACAGAGTATATTCAATAAATGTATGTTGATTGATG
  TATTGATTTATATTTTAATTAACCTGGAAGCATTGTTTTGACTCAAAGTGTTTGCAATTATAGTTTGGC
  AGATACCATATTTTTGCTAATGCATAGAAACACAAATATAACGGTATGCCAAGACAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_016593.5
• Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein is involved in the conversion of cholesterol to bile acids. Its substrates include the oxysterols 25-hydroxycholesterol, 27-hydroxycholesterol and 24-hydroxycholesterol. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
• Locus ID: 51302
• MW: 32.2