CNTF Receptor alpha (CNTFR) (NM_001842) Human 3' UTR Clone

CAT#: SC208546

3' UTR clone of ciliary neurotrophic factor receptor (CNTFR) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CNTF Receptor alpha
• ACCN: NM_001842
• Insert Size: 668 bp
• Sequence Data:
  Insert Sequence

  >SC208546 3’UTR clone of NM_001842
  The sequence shown below is from the reference sequence of NM_001842. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GCCGCCACTGCCAGCAGTCTCTTGATCTGAGCCCGGCACCCCATGAGGACATGCAGAGCACCTGCAGAG
  GAGCAGGAGGCCGGAGCTGAGCCTGCAGACCCCGGTTTCTATTTTGCACACGGGCAGGAGGACCTTTTG
  CATTCTCTTCAGACACAATTTGTGGAGACCCCGGCGGGCCCGGGCCTGCCGCCCCCCAGCCCTGCCGCA
  CCAAGCTGGCCCTCCTTCCTCCCTCAGGGGAGGTGGGCCATGCAGCTAACCCACCCACCAAAGACCCCC
  TCACCCTGGCCCCTTGGGCTGGACCCTCCAATGCCAGCGACTCCCAGGAGCCCTTGGGGGACGTGAGGG
  GAGCCTCTCACATCCGATTTCTCCTCCTGCCCCAGCCTCCTGTCTATCCCAGGGTCTCTGTTGCCACCA
  TCAGATTATAAGCTCCTGATGCTGGGGGGGCCCAGCCATCCCCCTCCCCCCAGCACCCACAATTTTCAG
  TCCCCTCCCCTCTGCCCTGTTTTGTATACCCCTCCCCTGACCCTGCTCCTATCCCACAGTATTTAATGC
  CCTGTCAGTCCCTTCTAGTCTGACTCAATGGTAACTTGCTGTATTTGAATTTTTTATAGATGTATATAC
  AGGGTGGGGGGAGTGGGCGGTTCTCATTAAACGTCACCATTTCATGA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_001842.5
• Summary: This gene encodes a member of the type 1 cytokine receptor family. The encoded protein is the ligand-specific component of a tripartite receptor for ciliary neurotrophic factor, which plays a critical role in neuronal cell survival, differentiation and gene expression. Binding of ciliary neurotrophic factor to the encoded protein recruits the transmembrane components of the receptor, gp130 and leukemia inhibitory factor receptor, facilitating signal transduction. Single nucleotide polymorphisms in this gene may be associated with variations in muscle strength, as well as early onset of eating disorders. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2011]
• Locus ID: 1271
• MW: 23.7