FUCA1 (NM_000147) Human 3' UTR Clone

CAT#: SC208483

3' UTR clone of fucosidase alpha-L- 1 tissue (FUCA1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: FUCA1
• Synonyms: FUCA
• ACCN: NM_000147
• Insert Size: 668 bp
• Sequence Data:
  Insert Sequence

  >SC208483 3’UTR clone of NM_000147
  The sequence shown below is from the reference sequence of NM_000147. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  TGGACTATAAAGCTGACAGGAGTGAAGTAATCATTTGAGTGCAAGAAGAAAGAGGCGCTGCTCACTGTT
  TTCCTGCTTCAGTTTTTCTCTTATAGTACCATCACTATAATCAACGAACTTCTCTTCTCCACCCAGAGA
  TGGCTTTTCCAACACATTTTAATTAAAGGAACTGAGTACATTACCCTGATGTCTAAATGGACCAAAGAT
  CTGAGATCCATTGTGATTATATCTGTATCAGGTCAGCAGAAGAAGGAACTGAGCAGTTGAACTCTGAGT
  TCATCAATTGTAATATTTGGAAATTATCTACAATGGAATCTTCCCTCTGTTCTCTGATAACCTACTTGC
  TTACTCAATGCCTTTAAGCCAAGTCACCCTGTTGCCTATGGGAGGAGGTGGAAGGATTTGGCAAGCTCA
  ACCACATGCTATTTAGTTAGCATCAGTTGTCACCAACAGTCTTTCTGCAAAGGGCAGGAGAGCTTTGGG
  GGAAAGGAAAAGGCTTACCAGGCTGCTATGGTCAACTCTTCAGAAATTTTCAGAGCAATCTAAAAGCGC
  CAAAATTCGCTATGTTTACAGTGATACTATTAAGAAAATGAATGTGATTCTGCTCTGTCTTTTTAAGTA
  TGATCAAATAAAAAATTTGTACATCACAATCATTTCTACCAAAAAAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000147.5
• Summary: The protein encoded by this gene is a lysosomal enzyme involved in the degradation of fucose-containing glycoproteins and glycolipids. Mutations in this gene are associated with fucosidosis (FUCA1D), which is an autosomal recessive lysosomal storage disease. A pseudogene of this locus is present on chr 2.[provided by RefSeq, Oct 2009]
• Locus ID: 2517
• MW: 25.6