HERC6 (NM_001165136) Human 3' UTR Clone

CAT#: SC208355

3' UTR clone of hect domain and RLD 6 (HERC6) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: HERC6
• ACCN: NM_001165136
• Insert Size: 667 bp
• Sequence Data:
  Insert Sequence

  >SC208355 3’UTR clone of NM_001165136
  The sequence shown below is from the reference sequence of NM_001165136. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  TTTGTCTCACCCATGCTCACACAGTCATAATCACCTCTGAGAGACTCAGGGTGGGCTTTCTCACACTTG
  GATCCTTCTGTTCTTCCTTACACCTAAATAATACAAGAGATTAATGAATAGTGGTTAGAAGTAGTTGAG
  GGAGAGATTGGGGGAATGGGGAGATGATGATGATGGTCAAAGGGTGCAAAATCTCACACAAGACTGAGG
  CAGGAGAATAGGGTACAGAGATAGGGATCTAAGGATGACTTGGACACACTCCCTGGCACTGAAGAGTCT
  GAACACTGGCCTGTGATTGGTCCATTCCAGGACCTTCATTTGCATAAGGTATCAAACCACATCAGCCTC
  TGATTGGCCATGGGCCAGACCTGCACTCTGGCCAATGATTGGTTCATTCCAGGACATTCATTTGCATAA
  GGAGTCAAACCACACCAGTCTTGGATTGGCTGTGAGCCAATTCACCTCAGTCTCTAATTGGCTGTGAGT
  CAGTCTTTCATTTACATAGGGTGTAACCATCAAGAAACCTCTACAGGGTACTTAAGCCCCAGAAGATTT
  TGCTACCAGGGCTCTTGAGCCACTTGCTCTAGCCCACTCCCACCCTGTGGAATGTACTTTCACTTTTGC
  TGCTTCACTGCCTTGTGCTCCAATAAATCCACTCCTTCACCACCCA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001165136.2
• Summary: HERC6 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]).[supplied by OMIM, Mar 2008]
• Locus ID: 55008
• MW: 24.8