ARAP1 (NM_015242) Human 3' UTR Clone

CAT#: SC208113

3' UTR clone of ArfGAP with RhoGAP domain ankyrin repeat and PH domain 1 (ARAP1) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ARAP1
• Synonyms: CENTD2
• ACCN: NM_015242
• Insert Size: 625 bp
• Sequence Data:
  Insert Sequence

  >SC208113 3’UTR clone of NM_015242
  The sequence shown below is from the reference sequence of NM_015242. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GACCCTCTGTCTCTTCTGCGCAACGTCTGAGCACAGGAGCCCATCCTTGGCTCTAGGATTCCGCCGCTG
  GAAGCCTTCTGTTCAGACACCCCTTATGCTCCAAGGCCTGATGTGAGCCAGCGGGGGGTGCATGGGAAA
  CTGCACCCCACAACCCACATCCTCCATCCTGACTGCAGCATGGGGTTCCCCGGCAGGGGTGGGAGGCAG
  CAGGGGTCAGCCTGGGCAGGAACCTCTCCCAACTCTGTCCAGGTGTTCAGACCTCTTGGCCCAACCTGC
  TCACCCCACCGGGTTCACTGTCCTTGTGGGGCTGGAGAGATGGGCATAAGTCAGGAACTTGGGAGGACC
  ACCACCCTTTCAGAGGCGTGAGGCCCTGGGGCCCTGCCGGAAGGGAGCCCCCTGCTCCTCCCAACAAAC
  TCCAGAACAGCAGAAAGCGGGTGCTGTAGAGGAGCACTCAGCTCACGGGGAGGGAGCTCTTGGCTGAGC
  TTCTACAGGGCTGAGAGCTGCGCTTTGGGGACTTCAGCCTTCCTTCCAGTCTGGGCTGAGTGGGGGGTC
  CAGACTACCTGATGCCCCTTCCCCAATTTGGGGACTTTTTGATAATATAAATATATCTGTATATTTTAC
  CCTA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_015242.5
• Summary: The protein encoded by this gene contains SAM, ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology (PH) domains. In vitro, this protein displays RHO-GAP and phosphatidylinositol (3,4,5) trisphosphate (PIP3)-dependent ARF-GAP activity. The encoded protein associates with the Golgi, and the ARF-GAP activity mediates changes in the Golgi and the formation of filopodia. It is thought to regulate the cell-specific trafficking of a receptor protein involved in apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]
• Locus ID: 116985
• MW: 22.2