Macro H2A.2 (H2AFY2) (NM_018649) Human 3' UTR Clone

CAT#: SC207974

3' UTR clone of H2A histone family member Y2 (H2AFY2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: MACROH2A2
• Synonyms: H2AFY2
• ACCN: NM_018649
• Insert Size: 629 bp
• Sequence Data:
  Insert Sequence

  >SC207974 3’UTR clone of NM_018649
  The sequence shown below is from the reference sequence of NM_018649. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CAGGAGATGGCCAAGCTCGACGCCAAGTAGCCGCCGCACTTTCCAGCAGGGATCGGAGGACGACCCGAG
  TCCCAAGAGTGGGGTTTTGCTTTTTAAAAGGAGAGAGGAGGGGTGATGGCAGGGGAGTGGAGGGTGGCC
  GGGCAGGTCCTGCCGGCGCAGGGAGCCCTCTGCCCTTCACACTCTCCTCCAAAAGAGCCTCCATCTGTA
  AGGAAGCAGGTCTCCGCGAGGGGTTTCTTTCCATGTGTTTTCCTCCTGTTGTTTTAGAACTTTTTTAAA
  AAAACAGACCTCGTTTTAGATTTATAGCATTGACTTTTACACACATTCACACAAGAAAAAAATCCTTTC
  AAAATTCTTAAATCTTCTGTTCCTCCTTTTTCCAAGGGAAGAGGGCAAAAAGTGGCCTGGGCTCTGTTG
  GTGTGCGTGTTCCGTGGCGGAGAGAAGAAAATGGGAAAGACATCTCACTGGTGCTTTTCTCTTTTGTTT
  TAGTGCCCCCCGCCCCCATCCCTATAATATCTGTAACTACTCCTAAAAAGGTTTTGATTCAGGCTTTTT
  TTTGGTTTCATTTTGTTTTTTTAAGAAAAAGAAAATGAAAGGAAAAAAATAAAAGATCCAGTGTCTTTC
  TTACAACA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_018649.3
• Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and may participate in stable X chromosome inactivation. [provided by RefSeq, Oct 2015]
• Locus ID: 55506
• MW: 23.8