Dishevelled 2 (DVL2) (NM_004422) Human 3' UTR Clone

CAT#: SC207417

3' UTR clone of dishevelled dsh homolog 2 (Drosophila) (DVL2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: DVL2
• ACCN: NM_004422
• Insert Size: 553 bp
• Sequence Data:
  Insert Sequence

  >SC207417 3’UTR clone of NM_004422
  The sequence shown below is from the reference sequence of NM_004422. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CCCAGCGAGTTCTTTGTGGATGTTATGTAGCCCACTGTGGGGCCAGGCTGGGCCGGGCGCTCCTGGTGT
  GTGACTGGGTGTCCTGGCCGTCATGTGCTTGCTCTTACAGTGCCTGGGCTCAGCCTACCAGCTGCTGCC
  ATACAGGAGATTGTGGCCACTGTGACTCTCACCAGCAGTGCCTGGTTCCTCCCCCTTCCCTCAGGGGTA
  GACAAGGGACCTTTGATTATTTTTAGCTTTGTTTTTTTATAAGCCTTTTTGGGGGTTAAAATAGAGTTT
  CTTACATTTTTGGGACTTTTTTAATAGGCATTTCCTCTTTTATATGAAGAATTCCCATCCATTGGGCCC
  CTTCTAACCCCAGAATGTGACCTCCTCCTCCAGTTACCCACAGCCCTGCCCTTTGCAGGGTTGGGGGTG
  GTCAGCGGTACCCCGGGGTTAGGCATCCTAGACAGCAGCCTGAGGAAGCTGGGAGATTTGGGCCATGTA
  GCTGCCTTTGTTACTCTATTTATTTTAGTCACTTGTATAAAACACCAAATAAAGCAATAGAGGCAAACTC
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004422.3
• Summary: This gene encodes a member of the dishevelled (dsh) protein family. The vertebrate dsh proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. This gene encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein, which may play a role in the signal transduction pathway mediated by multiple Wnt proteins. The mechanisms of dishevelled function in Wnt signaling are likely to be conserved among metazoans. [provided by RefSeq, Jul 2008]
• Locus ID: 1856
• MW: 19.6