KCNN3 (NM_170782) Human 3' UTR Clone

CAT#: SC207364

3' UTR clone of potassium intermediate/small conductance calcium-activated channel subfamily N member 3 (KCNN3) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: KCNN3
• Synonyms: hSK3; KCa2.3; SK3; SKCA3; ZLS3
• ACCN: NM_170782
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC207364 3’UTR clone of NM_170782
  The sequence shown below is from the reference sequence of NM_170782. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  ACCCCGTACACAAGTTCAAGCAGTTGCTAAATAAATCTCCCCACTCCAGAAGCATTACCCATAGGTCTT
  AAGATGCAAATCAACTCTCTCCTGGTCGCTTTGCCATCAAGAAACATTCAGACCAGGGAACGGAAAGAA
  GAGAGACCGAGCTAATTAACTAACTCATGTTCATTCAGCGTGCTTGGTCCGACATGCCTTGAAACCAGA
  AATCTAATCTCTGTTTAGGTGCCTCTACTTGGGAGCGGGAAGAGGAGATGACAGGAAGCGACGCCTCTG
  GCAGGGCCCTTGCTGCAGAGTTGGTGGAGAACAGAAATCCACGCTCAATCTCAGGTCTTCACGCGGGGG
  GTGGGGGTCAGATGCACTGAAGTAGCCAACAGCGAAGCCAGTCCAGAAGAGGGGTCCGCTGGGAGGGAG
  GGTTGTGTCAGGCTTGGGGGATGGGCTCTTCGCCATGGGGGTCTTTGAACACACCTCTCTCCTTTCCTT
  TTGTCTACGGAAGCCTCTGGGTGACAAAAGTAAAAGAGAGCTGCCCACAACTTGCCAAAACAGATATAC
  TCGAATCAGACTGAAAAAAAAAAAAAAAGACACAGACAAATAAAAAGCCAGATTTTCCACTCGATATTA
  ATACCCACATAAACCTGTGTGTTTGCAAACGTGTACATGTACACACATACACATCCCACGTTCGCTTCA
  GGTCCTTTCTTATTTGAGCTTAATCCAAATAAAAAGGGACTTGACACCTTACCCTGCATACAATAGGCA
  CCCCTTACATGTGTTTTGAGTTGGTCTGAATCTGAACATGGGGTGCTTTCAGTTCAGGTAGTTAGCTAG
  TTCTGGCCACATTCTGAGTTTCACTGAAGATGTGGATCCCTTCAGACATAATGCACATTGCTTTGTCCT
  GGATATGCACCTTGCTTGATTTGAAATGGATGCCAAGCCAAAATTGTTGGCATTCAGGAGGGATAAGCA
  GGCTTCTAAAAATAACAGCATCTGCAGAGTTTTCTTCTTCCATCCAAACAAGTTGTGTTTCGATGGTCC
  ACATGACCAGGTGTATGTCTGTAAGTGTGGAGGGAGAGGACAAGAAATTGTGCATGTGTGTGCAGACAT
  GCACAAACAGGAGCAATCCAATAACACCTTAGTGAATAGAAATATGGTTGGGGATTTGCTGAGCTGTAT
  TTATCCAGCAACAGGTTTCCAGCCCCAGATGTTAGTAGTGCAAAAGGGCCAAGTGCCTCAATTGTGAGC
  CTCTGAGCTAGGAGGAGAAGTGATGAAGAGTGGCCTATGTGGTCCCTTCTACCTGACCTTAAGTCATCT
  CAAAATGAAATATTGTGAGAATGAAGGGAACCCTTAGGGAACCTTGTGGGTAAGGTAAGTGGACATGGA
  TTTGTCAGTAGCCTGTTCCTACTGTGCCATGTTAATCTTGGTGCGAAAAACTATTCCAATACATCTCAA
  ACTCCAAGAGACTTCAGAAACATCAAGATTTAGTGTAATGAGCGGCGCAGAAAAATGTTTTCATTGCTC
  CATAATCTGACCACACGTAACATTTGTGACGTGAAAACCATGACTTTCTCATTCTGAGGTCTTTGGTTT
  CTGCCTGTGGGAAATTGAATGGCACTGTATGGACTATTTCATCTGTTGATGGTAAAACAAAAGGGTGAT
  TTTTTTGCTTGTGGTTGTCATCTTGGGTTTACCTTTGTAAGAAGACATCATCACCATTCTGAAGGCAGT
  GGCTTGGCATGGAGATTTTTATTCTGTAGCACTGGGCCTGTTCTTCTAAGGACAGCACAAGGTAGACAA
  TTGTAGAGCCAAGGCCACTTTTTCAGGAAGATCTAGTCTCTTGCTAACCCTCTTCTTTCTCTCTTGCTA
  TTGCTGCTGCTCTTTTGATGGTTTATAGTCTTAATGGCCTGCCTTGATAATTCCTTTGAACACTTTCAT
  TAGTTGTTTTGCATACCAGAGATGCCGCACCTGCTGTTGGCTTATTTTTTTACTTGTTCTATTAACTG
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_170782.3
• Summary: Action potentials in vertebrate neurons are followed by an afterhyperpolarization (AHP) that may persist for several seconds and may have profound consequences for the firing pattern of the neuron. Each component of the AHP is kinetically distinct and is mediated by different calcium-activated potassium channels. This gene belongs to the KCNN family of potassium channels. It encodes an integral membrane protein that forms a voltage-independent calcium-activated channel, which is thought to regulate neuronal excitability by contributing to the slow component of synaptic AHP. This gene contains two CAG repeat regions in the coding sequence. It was thought that expansion of one or both of these repeats could lead to an increased susceptibility to schizophrenia or bipolar disorder, but studies indicate that this is probably not the case. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]
• Locus ID: 3782
• MW: 74.8