Complement C8A (C8A) (NM_000562) Human 3' UTR Clone

CAT#: SC206814

3' UTR clone of complement component 8 alpha polypeptide (C8A) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: C8A
• ACCN: NM_000562
• Insert Size: 537 bp
• Sequence Data:
  Insert Sequence

  >SC206814 3’UTR clone of NM_000562
  The sequence shown below is from the reference sequence of NM_000562. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GGGCGGAAAGTACAGACGCAGGCTTGCTGAGGGCCTCTGGACACAGGCTGGACCAGATGCTGTGGATGT
  CGACCCCTGCACTGACTATTGGATAAAGACTTCTTTCAACTAAGAGAAGATGCAAATCAGCACACTTTT
  TTCTTTGTTCTGCCAGCTTCCAGGCCTAAGACTAGGTTTTGCTGTCTACAGCCAACTATTCTATTAGTT
  ACAAAACTCAATCATTTTATTCAGCAACTGGATGTTGACTGTTAACTAGAAGCTCTGTCCTACTTACAG
  CACTTTGGATCATCAAAAAAATAAAGTAAAATAGAAAACTGAGAAAACTCAATCCATGACCAGGGAGAA
  CTTACAGGATGTTAGAGACAAAACAAGCAGACACCTGAAACAATCAACGCCCAATAAAACAAAGTAGGA
  TGAAAATTCTCTTAGTTCTTTGATAACAATTTGTTCACTCATAGAAACATTATTAATTGGTAGGGTAAG
  CAGACACTCTGAAACAATGAGAAAAATACTAAAAATTGACTTGAGTTATTTCAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000562.3
• Summary: C8 is a component of the complement system and contains three polypeptides, alpha, beta and gamma. This gene encodes the alpha subunit of C8. C8 participates in the formation of the membrane attack complex (MAC). The MAC assembles on bacterial membranes to form a pore, permitting disruption of bacterial membrane organization. Mutations in this gene cause complement C8 alpha-gamma deficiency. [provided by RefSeq, Nov 2008]
• Locus ID: 731
• MW: 20.7