HIBCH (NM_014362) Human 3' UTR Clone

CAT#: SC206764

3' UTR clone of 3-hydroxyisobutyryl-Coenzyme A hydrolase (HIBCH) nuclear gene encoding mitochondrial protein transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: HIBCH
• Synonyms: HIBYLCOAH
• ACCN: NM_014362
• Insert Size: 1227 bp
• Sequence Data:
  Insert Sequence

  >SC206764 3’UTR clone of NM_014362
  The sequence shown below is from the reference sequence of NM_014362. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  TCTTTGGGAAGCAGTGATTTGAAATTTTGAGGTGACAGGCTTTTAAGGTATATTTTGTAGCATGGGTTG
  GCAATCTACAGCATGTGGGCCAAATCCAGCCTGCTGCCTGTTTTTATATACCCTGTAAGCTAAGAATGG
  TTTCCGCATTTTTAAATGGTTGGGAAAAGAAATCAAAGACTAATAATTCATGACGTGAAAATTATCAGA
  ATTCACAAATAAAGCTTTATTGGAACTAGCTATACTCATCTGTTTATATATTATCTGTGGCTGCTTTGA
  AATGAGTAGTTGCAATAGAGATGGTAAAGCCTACAAAGCCTAATTATTTACTGTCTGGTTTTTGTCAGA
  AAAAAGTTTGTCAATCCTTGTTTTAGAAGATGGAAAAATGTGAAGATCTTTGGAGATTCTCTTGAGTGG
  TATATCTAATTGAAATGGGATCTTCGTTTGGCTTGTATGTTGATGAAATCAACTTAGGTATACAATATA
  AAAAATAAAGACCCTGAAAATTGTTTTGGATACTGTGTTAATGCCTCATAGAATTTTTGTTTTGGATAC
  TGTGTTAATGCCTCATAGAATTTTCTCCATATATGAGTGTGTTTTAAAGTAACTGAGTTACTGTAGCAG
  AAGAGCTCCTTTTATAAACATTAGAAACCTTGGGCAGAGAGAAAAGATTTTTATTAACGTCCCTCTTCA
  GTATTAACTCAGACTTGGTCTTCCCTAAAATAATCAGAATACCATCCCATGGTGAAACTGAAAGGATTA
  TTTTCTTGTTATTTGTGATATTTGTTAATCTGGTAGAATGAGAAATTAGGAAACTCCAATCTCTTAGAT
  AGAGACACTCTGTTTTTTTATAATTTACGTAAGACTTTTGTGTGATAATTATCCTTACAAACAAGGTTC
  AAGTCAGGAGAAAAAAATTAATTGAAAGCAAGAAGACTTTTGTTCAAAAGATATATTCAATAATTGATA
  TTGTTAATGAAGTAAGTACTTTTTAAGGTATGAAAGAACTAAAAGGTATTCCAGACTCAGAACCTTAAA
  GTTCTAGGCAAGAATAATTTTTAAACTAAAGGTAAGTTTATAAATTAACAAAGTAAACAAAATTTATGA
  TTTTGTTTTTTGTTTGTAATTTTTGTTTAATTTTAAAGGTAAGTTTATAAATTAACAAACAAAATTTAT
  AATTTTTTGTTTGTAATTTTTGTTTATAAACAAAATAAATTTTTTGTAACTTGT
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_014362.4
• Summary: This gene encodes the enzyme responsible for hydrolysis of both HIBYL-CoA and beta-hydroxypropionyl-CoA. Mutations in this gene have been associated with 3-hyroxyisobutyryl-CoA hydrolase deficiency. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
• Locus ID: 26275
• MW: 49.1