NDUFS2 (NM_001166159) Human 3' UTR Clone

CAT#: SC206362

3' UTR clone of NADH dehydrogenase (ubiquinone) Fe-S protein 2 49kDa (NADH-coenzyme Q reductase) (NDUFS2) nuclear gene encoding mitochondrial protein transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: NDUFS2
• Synonyms: CI-49; MC1DN6
• ACCN: NM_001166159
• Insert Size: 484 bp
• Sequence Data:
  Insert Sequence

  >SC206362 3’UTR clone of NM_001166159
  The sequence shown below is from the reference sequence of NM_001166159. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GCCATCATAGGTACGAGGCCTATTGTGTAGTAGAGGTATCCTAGACAAAGGAGTTCGGGACGCCCACTG
  GGGACAGAAGGAGAACACTTCCTGTTCACCATAGGCCATGGCATGGACTCGGGTCCTCAATCTTTTGAG
  CACAGTAATGGGTTCTGGATCTTGGGTAACACCACTTTTTTTGTTTGTTTTGCCTCACAACAGGAAGAT
  AAGTAACATCACTTTTTTCCTCCATCCTCTCACCTAGGTACCCAAGATATTGTATTTGGAGAAGTAGAT
  CGGTGAGCAGGGGAGCAGCGTTTGATCCCCCCTGCCTATCAGCTTCTTCTGTGGAGCCTGTTCCTCACT
  GGAAATTGGCCTCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATGTTCATGTACACTTGGCTGTCAG
  GCTTTCTGTGCATGTACTAAAAAAGGAGAAATTATAATAAATTAGCCGTCTTGCGGCCCCTAGGCCTAAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001166159.2
• Summary: The protein encoded by this gene is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). Mammalian mitochondrial complex I is composed of at least 43 different subunits, 7 of which are encoded by the mitochondrial genome, and the rest are the products of nuclear genes. The iron-sulfur protein fraction of complex I is made up of 7 subunits, including this gene product. Complex I catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mutations in this gene are associated with mitochondrial complex I deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]
• Locus ID: 4720
• MW: 17.6