Synaptotagmin V (SYT5) (NM_003180) Human 3' UTR Clone

CAT#: SC205526

3' UTR clone of synaptotagmin V (SYT5) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: SYT5
• ACCN: NM_003180
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC205526 3’UTR clone of NM_003180
  The sequence shown below is from the reference sequence of NM_003180. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GACCGAGTGAGGCTGCTGCCTGCGCCCTGACTCCCACCCCAAGCCCCCGGCCAAGCCTGGACTCTAGCC
  CCTGACCCCAGACTCCCGAGACCGGCCAAAGCCAAGCCAGCCCCACCCTTAAGCTTCCTCTGACGGTTA
  CCCTCCCCCCACCTTCCACCCCAACCATCTGAACCATTCCTGTCTTCTCACCCCCAACGATGCCAATCA
  CGACAACTTTCCAGCACACTCCCCCGACCCTCGCGGACACACCCAGATGCCCCAGGGACCCCGGGAAGG
  GAAGGCAGCCTGGTTTCTCCTGCCCCCGGGTCCTGCCCCCTGCTTTGACAATCAGCCATCCTCGTCTGC
  TCTCCTCTCTCTTCCAACACAGCCCATCCCTGCACTGCTCCTGGGGCCAAATAAATATTCAGCAGCTCC
  GGTGGCCTGGGCCCATGCTCCTTGATGGGGGTGGGCAAGGGCTGGGACCCAAGAGGAAACCTCGTTTCC
  AGGAATGGGCGCTCGCAGGGCGGTGTGAGTGCTGTTCGTCCTGAAAGCCTCTGAGAGACTGGCCTGACC
  CCATGGACTCCCTCACACTCTCACCCGTTCATTCCTCACTCACCACTCTGAGCCTGGGTTTCCTCCTCT
  GGAGAATGTTGGGATAATAATAATTCCTGCCACACGCTGGCTGTGAGGAGTCCGCGAGAACTCCCTTGC
  TTGTCCATGAATTTATCCACTCGCTTGCTCACCCATGACTCACATCGATTTTCATACCCATTTCTGCAT
  CCCTCCCTCCCTCTCTTTTTTTTCTTCCTTCCATTCCATATTCCCTGAGGTCTTCTGTTGAGTGCTGGG
  GCCCCAGAATATCCAAACACAGACCTGACCCCCCAAGCGACTTACTGCCATCAAAACAAAGGAGAGGCG
  CGGAGCCAGGGAATACAATCTCCAGAGCCTGCGAGAGAGGTTGGAGTTCAGCAAGGAAAGCCAATGATT
  TCAACTTCACCTCTTTCTTTTTTCTTTTCTTTTTTCCTTTTTGTTTTCTTTCTGTCTTTCCTTTTCTTT
  CTTTTTCTCTTGTTTTTTTTTTGTTTGTTTGTTTTTTTGTTTTTGTTTTTGTTTTTTTGAGATGAAGTC
  TCACTTTGTCGCCCAGGCTGGAGTGCAGTGGCACGATTTCGGCTCACTGCAAGCTCCTCCTCCCGGGTT
  CACGCCATTCTCCTGCCTCAGCCTCCCGAGCAGCTGGGACTACAGGCGCCCGCCACCACACCCGGCTAA
  TTTTTTTGTATTTTTAGTAGAGACGGCATTTCACCGTGTTAGCCAGGATGATCTCGATCTCCTGACCTC
  GTGATCCGCCCGCCTCGGCCTCCCAATGTGCTAGGATTACAGGCGTGAGCCACCGCGCCCGGCCCTTCT
  TCTTTTCTTTTCTTTTCTATTTCTTTTCTTCTCTTCTTCTTTTTTTTAATTTTGAGACGGAGTTTCGCT
  GTTGTTGCCCAGAATGGAGGGCAATGGTGCAATCTCGGCTCACCGCAACCCCTGCCTCCCGGGTTCAAG
  CGATTCTTCTGCCTCAGCCTCTTCTTTCTTCTTTTTTTGACAGGGTTTCTCTGTGTACCCTGGCTGGAG
  TGCGGTGGGGCAATCATAGCTCACTGCAGCCTCAACCTCCTGGGCTCAGGGGATCCTCCTGCCTCAGAC
  TCCTGAGTAGCTGGGACCACAGGCACATGCAACCATGCCTAGCTAATTTTTTCTTTTCTTTTCTTTTCT
  TTTTTAGAGATGGGGTCTTGCTATGTTGCACAGGCTGGTTTCAAACTCCTGCAATCCTCCTGTGTGAGT
  AGCTGGGACTGTAGACATGAGCCGCCGCATCTGACCTCATTGTTCTTTCAACGAATGCTGCCTCCTACT
  CTGTTTCTAGCAAAGATGATAGTTCCATTGGCTTCTACCTTATCTCATCCCATACAGAATTTTGGGTGG
  TGAACAAAGACCTATAAGATTTGGCCAGAGAACTCTCTGAGCAGCAGATTAAGTGGAGAATGAGGCAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003180.3
• Summary: Synaptotagmins, such as SYT5, are a family of type III membrane proteins characterized by cytoplasmic repeats related to protein kinase C (see MIM 176960) regulatory (C2) domains, which are thought to bind calcium. Synaptotagmins may act both as negative regulators of vesicle fusion, allowing fusion in the presence of calcium, and as calcium receptors or sensor molecules (summary by Hudson and Birnbaum, 1995 [PubMed 7597049]).[supplied by OMIM, Feb 2011]
• Locus ID: 6861
• MW: 72.8