AKR1C2 (NM_205845) Human 3' UTR Clone

CAT#: SC203869

3' UTR clone of aldo-keto reductase family 1 member C2 (dihydrodiol dehydrogenase 2; bile acid binding protein; 3-alpha hydroxysteroid dehydrogenase type III) (AKR1C2) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: AKR1C2
• Synonyms: AKR1C-pseudo; BABP; DD; DD-2; DD/BABP; DD2; DDH2; HAKRD; HBAB; MCDR2; SRXY8; TDD
• ACCN: NM_205845
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC203869 3’UTR clone of NM_205845
  The sequence shown below is from the reference sequence of NM_205845. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CCTAATTATCCATTTTCTGATGAATATTAACATGGAGGGCATTGCATGAGGTCTGCCAGAAGGCCCTGC
  GTGTGGATGGTGACACAGAGGATGGCTCTATGCTGGTGACTGGACACATCGCCTCTGGTTAAATCTCTC
  CTGCTTGGCGACTTCAGTAAGCTACAGCTAAGCCCATCGGCCGGAAAAGAAAGACAATAATTTTGTTTT
  TCATTTTGAAAAAATTAAATGCTCTCTCCTAAAGATTCTTCACCTACTTTGGTCTCCATAACTTCTATG
  TTTTCTCTCCTTCTGACACACTAGTGCCCCCAAATTGTGATTTGCCTATACGTTTAGGGCCGGGGTTGG
  AAGATGTTAACAACCATTTAAGATTCATTTCTGCAGTGGGAGTGGGTGGAGTTTCACCCTCTGGGAAAG
  GGGCAGGTGACAGGTATTTATCAGTCAGTGCCTCTCTAGCTCTTGTAGGAAGAAGCACACGCAGGATGG
  AGTCTAGAGGATGAGCGATATTGACTAGCAATTCATGGGCTCCCTCCAGCAGTGCGAGGGTCAGAGTTT
  CTGGAGCCTTGGGAGGAGGCATCCCTGTGAGGGGGGGTTAGGGAGATGGGAGGGCACCAGGAAAAGTGA
  TTAGAAGTCAGGTATGGGAAGGCTAAATAGGACAGAGTCGAGTACATCTCTGCTTGGAAAAACATATCA
  ACACCCTTTTTTTTGATCATTATATCTTGTTCATAAAAGAAAACTTTCCACATTGTTTTAACAAACCCC
  ACAGCTGAGAGTCAGGCCTGAATCTTTGATGTGTGCCCATTCACAACGTTGACCCTATTGGTTTGTGGT
  GGGGCAGGACATCGAAGATATCATTGACTCATCACATTCCCCTGAATAGCTCATATTTAGAAAATATTC
  TTAGATTGTAAAAATGTACTGTTCATTTGTTATATGCAATCTTTTAAATGTTTTATACTTTAAACAAGG
  CATAGTTACAAGTATAAAACATAAATATCCCAAAGCCATTATGCATGGCACTCAAGATTAAAATGGGAA
  ATAATACATCTAATAAATCAAATGTTCCAAGACTTCAAATGTCTTTTGGAAACAGGCTATGTAAAACAG
  CACACTGGTTTCAAACTTTGGTAAATTTTAAGAAGAACTCTTACAAAGGCATTTAATTCTTATACATAA
  TTTTCAGGGGAACTAATCAAATCAGCTAATCATGAAGACATGATTTTCGTTTTAGAAAACACTTTTGAA
  AACTTGGGATAATCTCATGTCTTAATGATCAAAGCATTATGAGAAGGACAGTGGTTTTTTACCTGGGCA
  CACTTTCTAACACATTTACTCTCCACTATTCGTACTCTGGTAGCCATGTTAACCCCATCAGAGATTCCT
  TCTCAAGCCATGTCTCAGAGCTGATAGGCATCCCAGCAAGTTTTGCAGCTCACAATTTTTCTGTAAATT
  ACTTATTCTATAAAATTGGAAGAGGCCATAAACTTTGGAGGGCCCTAGACCAATTTTTTGGATTATTTC
  TGGTCTACTCTCATTCCGTTGATGATCTTAGATATTCTCTGCATTAAATATCACCTCTAGGCTGAGAAA
  TCCACCAAAAAATATTTCTAGCTCAGCGTTTTCCTCCAAATCTTCAATGGAAGATCATAATGTGAACTC
  TGCATCTCCATGTTAAAGTTTAATGGACATTCACACTTAGCATGTCTCAAAGAAATCTCATGTAAACCA
  TGGCCATCCTGTTCTACCTTAACTTTCTGAGTCTATGGAATGATAATTTCACATCTCATAAACTTGACT
  GATGTAAGTGTCAAGAAAAGATTGACATTTTGTTAAAACTTCGTAGCCAAGTGTGTAACGCTTAAGCAG
  ACTTTCATATTTCAAATCTCTATAGCACGTGTAACTCTTTTTTCAAGATGTGAAATAATCATTAGGTCA
  GTCATTTGTAAATAGTACGGCTGCTGTGGGCTTTTTCCAGTTCTTCACCATCCATTTTTATAAAACTC
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_205845.3
• Summary: This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
• Locus ID: 1646
• MW: 76.6