Apc1 (ANAPC1) (NM_022662) Human 3' UTR Clone

CAT#: SC203043

3' UTR clone of anaphase promoting complex subunit 1 (ANAPC1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: Apc1
• Synonyms: APC1; MCPR; TSG24
• ACCN: NM_022662
• Insert Size: 1681 bp
• Sequence Data:
  Insert Sequence

  >SC203043 3’UTR clone of NM_022662
  The sequence shown below is from the reference sequence of NM_022662. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CTTGGAAATCCACAGCCAATGGTGATGTGACCGTGTCTGGCGGTGAACCTACCCTGAAACGTGACTTCT
  GCACAACAAACGTGACCAAACATCAAAGCTAAAGCAATGTTTATAAAGTTTTATGGTATAACTAGGGGG
  AAATGAGCTGCACAAACCTCAATGTATTTTAAATCTGTTGCTGTCATCATTAACGGTATATGACATATA
  AAAGCAAGTTAAAATTTACTTTTGTAAATAAAGTTTTTGGTTTGTTTCCAAAACTCTTGATGATTGCTT
  TAGTTTTGGACTTAGAGAATAGAGCAGGGGTTGCTGGAGTGAATATTGATTTTTAAAGTCTTTGAACTG
  TGGTGGTATAGGTGAAGTGACTATGCCCAAAAATGCCAAGTTTTAAAAGAAGCTATGTCATAAAGTTTT
  ACTTTCTGTGGCAAAAGAGCGCTTTAGCATTTCCTCAGATGTCACAGTTGTCCTGTCTAAAATAAGTTT
  GTACTTCTGGTGACAATGCCAGACACTCTTATGATGTGATCACCTTAAACAGGAGAAGGAGATTTTTGC
  CCTCAGTTGCTCAACATGAAGTACTGTGGACTGAATTGTGTCCTCCCAGAATTATATATTGAGGCCCTA
  ATCCCTGTGTGACTGTATTAGGCAATAGGGCATATAGGAGATTACTAAGGTTAAGTGAGGTCATCATAA
  GGGTGGGGCCTTAATTCAGTAGAATTGGTGGCCTCCTAAGCAGAGGAAGAGAGATTTTTCTTTCTCTCT
  CTGCCATGTGAAGACAGTGAGGAGTCGGCCGTCTGCAAGCCAAGAAGAGCCCTTATCAGGAACAGACTT
  GGCTAGCACCTTCATCGTGGACCTCCAGCCTCCAGAATTGCAAGAAAATACATTTCCGTCGTTGAAACC
  ACCCAGTCTGTGGTATTTTGTTATGGCAGCCCAGGCAGACTAATACGTGAAGCCTGCTCTAAATAGATA
  AAATAAGAAATTACTACAGAGGGCTCTTTAGAAATTGTATTTAAAAACAAGACAATCCATATTTACCTA
  AGATTTACAGAATGTATGTCTATAAAAGGAGGGATTTCTGGACTAGATGATGATGAAAAATGTTCATAT
  AAAGGCACCTCCAGCTTCGAGTTGCCAACACAGGAGGAAGAATGCTCCCTGCTGTTCAGATGCTGATAT
  GTGTCCTGTGCTTCCTGGATGGCCAGTGGGATCATAAGCTGGTAGAAGCAAGACCTTCATCCACTGACT
  TCATATTTCTTCCACATCCTGAACTGTGGTGTTTGACTTTAAAAATAAATTAAAGCAAATAGAAATGTT
  TCCATTGAGATTTTGGCAAAAACCCACATGACATTTGCGTCTGCAGAGTAGACTTGGAAGGCATGCACC
  TGGAGGCCAAGTGGTATTCTCTGGACTCTCTGTCCTTGCATTTGCGTGGCTGCCCCGAGGACTCGCTTC
  TTCCTGGATGTGAAGCAGAGGCACGATGGGGCTGACTGAGAGGCTGAAATGTGCTTCTCTTCACAGTTA
  CATAGTTTTCTTCTAATTAAAAATAAATTTTAAGTGGTAGATTATGAAAATTCCATTTGAATCAAGATT
  TTAAAAATTCAATATCAAACCAGTTGTTACTGTCTTCACTCTCCGTTGTTTACGATTGTGTGCTTTTCC
  TGAATAAAGAATGACTGGAGAGACA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_022662.4
• Summary: This gene encodes a subunit of the anaphase-promoting complex. This complex is an E3 ubiquitin ligase that regulates progression through the metaphase to anaphase portion of the cell cycle by ubiquitinating proteins which targets them for degradation. [provided by RefSeq, Dec 2011]
• Locus ID: 64682
• MW: 63.7