JNK1 (MAPK8) (NM_139046) Human 3' UTR Clone

CAT#: SC203014

3' UTR clone of mitogen-activated protein kinase 8 (MAPK8) transcript variant JNK1-b1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: MAPK8
• Synonyms: JNK; JNK-46; JNK1; JNK1A2; JNK21B1/2; PRKM8; SAPK1; SAPK1c
• ACCN: NM_139046
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC203014 3’UTR clone of NM_139046
  The sequence shown below is from the reference sequence of NM_139046. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CCCTCTCCTTTAGCACAGGTGCAGCAGTGATCAATGGCTCTCAGCATCCATCATCATCGTCGTCTGTCA
  ATGATGTGTCTTCAATGTCAACAGATCCGACTTTGGCCTCTGATACAGACAGCAGTCTAGAAGCAGCAG
  CTGGGCCTCTGGGCTGCTGTAGATGACTACTTGGGCCATCGGGGGGTGGGAGGGATGGGGAGTCGGTTA
  GTCATTGATAGAACTACTTTGAAAACAATTCAGTGGTCTTATTTTTGGGTGATTTTTCAAAAAATGTAG
  AATTCATTTTGTAGTAAAGTAGTTTATTTTTTTTAATTTCAAGTGATGTAATTTAAAACCTAAGTTGTG
  TTTCAAAACAGCAACAAAACTGTATTGTATTTTTTTTGCTGTAATTAACTGTATAATGTAAACCTAATT
  ATTTTATCATGGTTTAAATTTTTTGCATATTTGCTTTATCTTATGCTGCTGATTTTTTTAACTGAATTT
  GTAAGATTTTGTTTATCAAAGCAACTATTATGTGGTGACTTGCCTATATCATGAATTATTTAAGATTTT
  TATAGTTTTTTTTAATTAGAATTTATTTCAGATGTTTTGTTCATGATACTATCCTTCAGGGTTATGTGC
  TTATCAATGAAATAACCCCAGAGGAGTGAGGGAAAATAACTTGTAGCCAGTTATATTCAGGAATAACTA
  CTGTAAATGATGAACGTGTTAGGAGACCTCCAATATTTGCTACTTGCCAATCCTAATTTAGTTACAAGA
  ATTGGTAGGCAATCCTACTTAATTTTGGCAAAAGCCCCGTCATCTAAATGGCAGAATAACTCAGAGCAT
  GTCTTTGAAGATGCTGGGCGTCTACCACCACCTTATGTCCCCACCCTACCCAACAAAAATAAGTAAAAA
  GAATATGGTGTATTCTACAAATTTGTGGCATGCTCAAAGTTTATGATCACATAAAGGCAAGAGGATACT
  TCATGAATAATACATTTCAATGCAAATAAACAGATGGTTCACTTCTACTAGCTATGAGCCTGTTTTTGT
  ATACACTGAGTTAATCTACTCAGGCTGTAGGTCCCAGCAATGTTCTAGAGTCTGGTCTTTCCCTTTCCT
  GCAGCTTCGGGTCCTTGGACCTTTCCTGTTTCCTATTACTTGGAGTGTCTGTCAGTTGAGCACCAGTTG
  TTCTGGTGTTTCATTTGATTCTACTTGTAGCATAATCATTTATACGAGCTATTGGGAGGTTCCAAACCC
  TACCTAGATTTGTGTAGGTGATGTATCAAATGAGCAATATACCGTTCATCTGAAAATAGTAGCACACAG
  CCATATATAGGATATCATTTTCTAAGGACTGTTTCTTCACATTGAGCAGAGCAGGCATAAATGGTGGTT
  ATTTAGTCTAAGTCTTTTATTTTTTTATACCTGATTTTCAACATAACACGCAATGTGGATGTCGAGTAG
  TGTTAAGAATGGTGCTGCTCCTGACAAGTGTATGTTAACTGTTTACATTTTCTATCTGTAGAATTATTT
  CTCTATTACTGAACTTTTCCTAAGTAAAATGTCTTTGAAGTCTCGTTATTTCTGAAATACGTTGTCTGT
  AATAGACCCAGGCACCTTTTAAATTATCTCTGGAACAAGAGGGATTTCATGTAATGAACTAGGAAATGC
  ATACTCACATAAGCAACAAGGTTCTAGGCAGAAAGCCCCTTGGAATTTGTGACCAACAGGAGCAAGAAC
  AGGTGCGGCTCAACATGCAATGTCTGAAAATTTGCTTGGCATTTTATTCATATATTTAGTGCAAAATTA
  TTTTTGAGTGAGATATTTTACATCACTGTTAATGTGCAATATTTAAGATTAAAATACATTAGCTTTTTT
  ATATACTTTGAAGTAGCAAGTTTGTTTTCGATGGCTTAGAGTCATGATTTCCAGCTTCCCAGCCTTTTT
  ATCAGTCCCTTTTCTAATACAACAAGGTGCATTAATTTGATTAGGCAAATTAGAGTTCTAAGACACTT
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_139046.4
• Summary: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Apr 2016]
• Locus ID: 5599
• MW: 78.4