ELOC (NM_005648) Human 3' UTR Clone
CAT#: SC202987
3' UTR clone of transcription elongation factor B (SIII) polypeptide 1 (15kDa elongin C) (TCEB1) for miRNA target validation
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Specifications
Product Data | |
Vector | pMirTarget |
Species | Human |
Transfection Reporter | RFP |
Assay Reporter | Luciferase |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Symbol | ELOC |
Synonyms | SIII; TCEB1 |
ACCN | NM_005648 |
Insert Size | 1541 bp |
Sequence Data |
>SC202987 3’UTR clone of NM_005648
The sequence shown below is from the reference sequence of NM_005648. The complete sequence of this clone may contain minor differences, such as SNPs. Blue=Stop Codon Red=Cloning site GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC CTGATGGCTGCGAACTTCTTAGATTGTTAAATAAAATAAATTATAATAAACTGTTAACTCTTTTCAGTA TTTAATACCTGTAGTTCAGTTAGTAACTTTTTCATATATAGCATGTTGCCTGTATGCAGTTGAACTATA TAAAGTTCATTGCAAAGCAGATTATCTTGTTTTTTTGCATAGCAATCAAAGTTGAAATTTGTTTGCTAC ATCAACAAATTAAGGACATTTTCACAAACTGAGAAATAAACAAATATGCCAATTCGTAGGTGGTTTTGC CTTATCCTTTGAATGTGACTTAAAATCAGCAATGATGATATAGTAAATACTGAAATTTAGGTGTAAATC AATACGTTCTACAGGGAAATAATGAGGCTAAGTATTTTTATGTTTTTAGTGGTTTTTTAGAAACCTAAT CTTATAGCCGCCATTAGCATTACTAGAGTTATGCAAATAATTGCATTATAAACATGTTTATAACTTAGC CAAAACATTGATTTTTATAACTCTCCAAGTATGAGTTGAAATTTCTTATGTCTTTTGATAAACTGCAGT ATTCTTTAAGTGTACTTTGTCCTTTTGTTTATTGCTACAATTTAAGAACTTTATTTAAAAACAATTTTG GAAGGTTACTAGGTACAGCTTTTGCAGAAGAAACTGAACTGTAGCCAGATGGTCAGCAAGTAAACTACA TGACTTAGAGTTCTTGTGCATTATACATTTTGGGTAATTCAAAGTACTGTATTTGCATTGACTATAAAG CGTCGTTTGCAATATTTATTCTTCCTTCATAGTCCCTTGTTGTGATTATACTTCCAGTTGTTTAAATGT ATTTTAAAAGACTCACTATTAACTTTATTCAGATCTTAAAAGCTACACCTTTTTGAACTATTCCAGTCT TTCTCCATCCAATTAGAATGTTATGGAATAACATGTGAAGAGATACAGTGAAAATCATATTATAATGCA TTGAAGATATCCTACATGAATTATCTTCTATTAAAATATGTCCCAATAAATATACCCATAATCAGTGGG TCTTCTACTGAAATCAAGCTCATTCAGCTTTTCTATTAAGCAGCCAAAAAATTTTTTGAAAAAATATGA CAGCATCATTCCTTATGCATGGGGAAAGAAGCACTGGAGATGGGTTTAGTATTGTCTTTATCTCAGAGG TGGAACAAAGTTGTCATTATAGGGTCTATAAACTTAAGTCTCCAGCTTATTTGAGACTGGCTTAATATG GCTGAGCCCGGTGGCTCATGCCTGTAATCCCAGCACTTTCAGAGGACGAGGCGGGTAGATCACTTGAGG CCAGGAGTTTGAGACCAGCCTGGCCACCATGGCAAAACCCCATCTCTACCAAAAATGCAAAAATTAGCT AGGCCATGGTGGCACACGCCTGTAATCCCAGCTACTTGTGAGGCTGAGGCATGAGAATCGCTTGAACCT GGGAGGTGGAGGTGGCAGTGAGCCGAGATCGCACCACTGCACTCCAGCCTGGGTGACAGAGCGAGACTG TCTCAAAAAAAAAAAAAAAAAAA ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG |
Restriction Sites | SgfI-MluI |
OTI Disclaimer | Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs). |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. |
Reference Data | |
RefSeq | NM_005648.4 |
Summary | This gene encodes the protein elongin C, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been identified. [provided by RefSeq, Mar 2011] |
Locus ID | 6921 |
MW | 59.6 |
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