XPD (ERCC2) (NM_000400) Human 3' UTR Clone

CAT#: SC202874

3' UTR clone of excision repair cross-complementing rodent repair deficiency complementation group 2 (ERCC2) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ERCC2
• Synonyms: COFS2; EM9; TFIIH; TTD; TTD1; XPD
• ACCN: NM_000400
• Insert Size: 1822 bp
• Sequence Data:
  Insert Sequence

  >SC202874 3’UTR clone of NM_000400
  The sequence shown below is from the reference sequence of NM_000400. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AGGATAGAGCAGATTGCTCAGCAGCTCTGAGTGGGGCGGGTGGGGCCATAAACGGTTCCTGGTGACTCC
  TGAGTCTTGCCTGGCCCTGGTTCCCAGCGGCGGTGGTGCTAGAAGGTCTTATGAAGTCAGGTGACATTT
  CTCACTGTCACGTCCACAGCCTTTAATCGCAGGAGAAGGCAGCTATCCACCAGGTACCCAGAGGCAAGG
  GGGGGCCAGGAGATGATAGACCCCCTCTCACCCCACCAGCCCATCCCTCCTGCACTGTTCCCAAGAAGC
  TGCGGCCAGCGCAGTTCACTGGACGTTAGTGGGGGCTCAGGTCCTGGGTGCTGGCGCTGAGGGTCCGAG
  GGGCCTTGTCCAGGTGCCAGCTGGGAAACTGGAGACAGGCACAGGGGATGGTTGGAGGGGAGGTGAGGG
  GCAAGTGTTACATCCACCCTCCAGCTCCAGGTCCAGCCTGGCAGCCAACTATCCAACCCCCTCGCCTCT
  ACCCCAAGTAAAACCCATCTTTGCTCCTGTCCTGGGTCTCTTCTGACACCAACCCACCACCTGCTGACT
  CCACCACTGGCCCGACCGCCCTGCCTTTGCCTCATCTCTGCACCTACCCACATGGCCTCCTCTATTTTT
  GACCCAGATGTCCCCCTCACCTGAGTCCCAGGAGCCCTTGGAGCATCCACGTTCAGTGTGTTGAGTGAC
  ATGGCTCTCTTCATTCTGCAAAGAGGGCAGCAGGGAGGAAATGAGTGAATCCAGGAGTGGCCCCCCTCC
  ACGAGGGACCTTTCCAGCACAGGGTTTGATCTGTGTGTATCACAGGGGAGATGGGAGCCATGGAAGGTT
  CTTGAGCAAGATGGGGGTGGGGGTGGGGTGGGCTGTGGATTCTGCTGATGTCAGGGCTCTTTGCCGACC
  TGATCAACACTCACCCGGCTGCTCCTGCCGCCGCCTCGCCTCGGAGCCGGGAGACCAGCTTCTCACTTC
  CTCGCCTGATAGACTCACGGATCTTGGAGAGTGAGCTGCTGCGGCGAAGGGCCTGGGGAGGGGCCGGGG
  ATGCTGCTCAGTCCCCCAGGCCCATGCAGTCCCCACGCCCACCCCAGGAGCCGAAGCACAGCCCATCCT
  CACCTGTTCTGCGTCACCAGCTGTGCCTGTGTTGGGGGCACCTAGGGGGAAAGATGGGGGACACTGAGA
  TGGGGACATAGAGAAGCCACCAGGAGGGAAGACAGACAGGGGCTAGGGGCTCACCGAGAGGGGCGGGTA
  GGTCCTCCTTGTGGAGGATTTCTTTGTACAGCTCTTCCGCTTGTTGATACTTGTTCTGTTTCAGGTAGG
  CTGAGGCCTGCGGAGAAGGGAGTGTCTGGGAACTTTTCGGACCCCAGTTCCCAACACTGCATCCAGTCC
  CGCCTTTTTTTTTTTTTTTGTAGAGACAGGGGTCTTGGTATGTTGCCCAGGCTGGTCTTGAACTCCTAG
  CCTCAAGTGATCCTCCTGCCTTGGCCTCCCAAAGTGTTGGGGTTACAAGCGTGAACCACCACGTCTGGC
  CCCACTTTCTAATGTGCTGACCTGGCCGTATCCTTCCCCTGCCTAAAGCCTTGCCCTGGGGCAGAGTGG
  GTTTGGAGCTCGGCCTTACTTCCTGCCCTGGGGATGGAGGACAGGCAGCCTCCTGTTTCTGAGCCTGTC
  TGCTGTACTTTGGGCTGGTGGCATGGCCTCGGTGCCTCAGTCTTCCCACACGCAAAGTGGAGCGACAGC
  TCCCACCTCATAGGGACGCTATAAGAACTAGCGATGTGGGGACCGGGCTAAGAGGGGAGCCGGCCACAG
  TGAGCTCAATAAATGTCTGCTGCTGCCA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000400.4
• Summary: The nucleotide excision repair pathway is a mechanism to repair damage to DNA. The protein encoded by this gene is involved in transcription-coupled nucleotide excision repair and is an integral member of the basal transcription factor BTF2/TFIIH complex. The gene product has ATP-dependent DNA helicase activity and belongs to the RAD3/XPD subfamily of helicases. Defects in this gene can result in three different disorders, the cancer-prone syndrome xeroderma pigmentosum complementation group D, trichothiodystrophy, and Cockayne syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
• Locus ID: 2068
• MW: 64.8