TBCE (NM_001079515) Human 3' UTR Clone

CAT#: SC202543

3' UTR clone of tubulin folding cofactor E (TBCE) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: TBCE
• Synonyms: HRD; KCS; KCS1; pac2; PEAMO
• ACCN: NM_001079515
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC202543 3’UTR clone of NM_001079515
  The sequence shown below is from the reference sequence of NM_001079515. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AATGGAGATTGTCTATTAGTGCGATGGTGACAACCAACTAATAAAATTTAAAGACCACACTGCTTATCG
  TGTCTGGGGTTCACCGGAAATAAATGATTCACTGGAACAATTCTACTGTCAAAACAAAGGGGGTTTACA
  ACTTGTCCTAAGTATAACAAGGGATGTATTTTTTGTTGGGAAGTGACCATTTCTAGGCTTATACATAAT
  AGCAATAATAAAGGCTTTGAACCTACTAATGATTTTCTGATCTTATTTCATATTTATTTTTACAGCTCA
  TCACTGCATTTCATGATAAGATTTAAATATTAAATAGAAAGAAACTAGCTAGCCTAATAAAATCTGAAC
  ACAGTTAATATCTGTCATAAGACTAGTTTTAATGGAATTCTCTATTGAAACTACTATTTTAAAGGGTTA
  CTAGAAATGATTTGGTTGGTCATTTTGGGAAATGTCCCTTAAACTTGGGGAGACATCCTCTACTATGTA
  TAACAATATGCTATTATCTGTCTTCTCAGTTGCACTATTTCTAAGAGTACTTAAATTAATCACATGCTT
  TTCCCTACAATTATACCTAAGCTGAGTATATCTTCTTCTGTGATAACCAGCTTTGATTGAAATGTACTC
  ATATTAGGTAAACATTAGGCAATGATAGGAGGAAAGCAAAACTAATTCTTTCAAAATGTCAACAAAATT
  TAGAAATATCCTTCCCGATGGCACTAAAACCCTGAGAGGTATTTGCTTTTATTCATACTCACACAACTT
  TAGCATTTAAAAACTATGAGTACTAAACTGTGACCTTCAGGATTTATGTTAGATGGCAGAAAGAAAATT
  TGGGTATTAGTCTACCATATAAATGAACTTCTTTAAAACCAAGGTTCAGAACTGAGAATCATATTGGTT
  CCTCTTCAAGTTAGTTCAAGTTGCCCACTTCAGAGATCCACAAAATCTGACATTATTTCCAGAAACCCC
  AAACTTTGGTATAAGTGACCACTGCTCAAATATGTGATCACATGATCACACAGCATTCCTGTGAGTTCC
  TTTTTGTCTGATAATTATCCTAATTAGCTCTACAGAGCTATCCTGCAATCCAGGTTGAAATTCAAACTC
  TCAGTTACTACTACAGATTTCTGAACTAGGCCAAGTTTTAACCAAAAACTATAGGTAATGGTGAATTAC
  TAATTAGCCACAATGCATCAGGATTTAGAAATATTTTTTCTTTTATAAAATAACTTGGTATTTTTCTGA
  TAATCTTCCAATAGATAAATAAAAACTTTTCTTATGCTACAGTACAAGTTGATTTTTAAGGAAATTTGT
  GCAAACATTAAGAAACACCGCATTGGTTCTGGGTGAAAGTGCCAGTCTGGAACTCTCTTGAAAGACCAT
  ACAGTCTACTGCTAAACCCTGGGACTCCTCAGACTTGCACTCAGATTATCGTTTGCCTGCCCTGATTTT
  AGACTCTGCTAATTCAAGTCCCTGTTATCTTGCTTGACATCGACAAGGATCACCGCACCGTTCCTTCAG
  TTTCCACAGTTCCGTCAGTTCCCACGGAGAATACTGAGGAGAAGACAGCATTCCTGTCTCACAGGTCTT
  CTCACACAGCCACAGACTGTCCTGTTGAGAAACAACCAAAGCCGATCTGAGAGTGGTGAAACTGTTTTA
  AGAGCATCAGAAAGTATGCACGTAGACAGCTTTTATGTATTCTAATGATGCTGAAATTATTTCAAGGAT
  AACTCCGTGTGTGGAACAACTGGTGAGGTTTGGGGGTGGCACCTCCTGATTTGGGAAAGCACCAGGTCC
  CACAGTCCTGTGGCTGTGGAATACAGAAACAAGGCGGTGTGTGGATGAAGAGTTAGTCAACAAATGCCA
  TTCCGTAATGAACGATTTTAGAAACCACAAGTGAGTTTCATGTTTGCTAGTAAAGGTGTGTCTATGGCA
  GTATTAGTAACAGCTATTATTCTGATGGAATGCTTACAATATTGCAGGGACTGTACCAAGTGCTTTGT
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001079515.3
• Summary: Cofactor E is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
• Locus ID: 6905
• MW: 77.2