Muscarinic Acetylcholine Receptor M3 (CHRM3) (NM_000740) Human 3' UTR Clone

CAT#: SC202331

3' UTR clone of cholinergic receptor muscarinic 3 (CHRM3) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CHRM3
• Synonyms: EGBRS; HM3; PBS
• ACCN: NM_000740
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC202331 3’UTR clone of NM_000740
  The sequence shown below is from the reference sequence of NM_000740. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CACAAGCGCGCACCCGAGCAGGCCTTGTAGAATGAGGTTGTATCAATAGCAGTGACAAAACGCACACAT
  CAACCCACAGACCTTAGGAGGAGGAAGGCGAGGGCGGGGTGACTTCTGGTGATGATAAAAATGGTTTTA
  TCACCCAGATGTGAAAGAAGCTGCCTGTTTACTGATCCATTGAATAAACCCATTTTAATAGAAAAAGTC
  AATACCAATTCAGCAAAAAGAAAAAAAAAACATACTACTGAATATAAAGAAATTTATTCTGAAATAGAC
  TTTACGTGTTTTTTTCTTAAAGAGGAGAAAAATATTGCTTGACGGCAATTATATACCCAAAGTGATTTG
  CCTGGGTCCTTTAATTCCCATTAGCTTTGGAATCTCAGATGAGCATAGCTGACCCAGTTCCCACATTCT
  TCCCAAGGATCCCAAAAGTGGGAATCCAGACCCCAAGTGGAACACTGCAGGCTTACGAATCTGTGGTTC
  CAAAATTATTTCATACGTTGCAAAGCTGAATCTTCTTGTCCCAATAGAGCTTCCTGTCTTTTCTTTGGT
  GTGTTGTTAAACTCTATTTGTGGACTTGATTCTTGATTCTTGCAAAGTACTGTTTTGTGCAGTTCAAGT
  TTCGTACAAATAAAAATACTTAAGTATATATATATGTGTGAGTTCTGCACGCACACACATAGTGTATAT
  AATATCATGGGAAACACTGAACTGGCAAATTATTCCTGCAACATACGCTTTCAGTACTTTGGTAACTGA
  AGTTCTCTAGGATCCTAATGCAACATTAACGTGAAATAAGCCCAGTGTAATGTTTTTGAAACCAGGGCT
  GTTTTCCACAGAGAGCAGCCAGGCCTTCCCAGCAGGTCTGTGCAGAGCGGACAGGCTCGTGAGTCAGCT
  GAGCGCCGTGGCTTCGCCAGACTTGGTGTTAAGCAACCTCCTTTGTTGATGTCTCAACAGAGCTAAATC
  GGGGCCCCTCTGAGCTCAAAGAATGAACCACATCCACACGTTTGAATTTAATCATCTAAATCTGAATGT
  TTCAGAACAAAATTTCTGCTATCTAAACTGCTTGAAACTCAATAATAGTGTCACGTTTGAATGTCATAC
  ACAGCAATATATATATATGTGTATATATATATATATGGCAAAGCAAAAAAAAAAACATGGTAAGAGAGA
  ATGAAGGAGAACATTGTGTTTGATTCTTGCTGAATGGCACCTTCTCAAAGAAAATAGGGCTTGCACCTT
  TGTTAATCAGCTGTGGCCAGTGCTTTCTGGTGTTCATTGTGTAACCTTCACCCAGGAATAGGTGAGGTT
  TTAGGAAGTTACATGTCCTCTGAAGAAAGAATTACACTCTGAAAAGTAATGCTTCAAATTGATTTCCTT
  ACCTTTTGGGAAAAAAAAAAAATTGTTTTTTTGCATTCTCCCTTGAATTGACCAAAATGTTAACTGTTT
  CATTTGGGGAGGGGATGGGGTGCTGCCATCATTGTCGTTGTTGTTGCTGCTGTAGCTGTTGGGGTTTCT
  TTTCCTGTTGCCGGGGCTGTTTGGGGAGAGGGAGGGGAGGGAGGTGGGAGGGCCGCGGAGATATCTTCC
  CTTTGTACAGGGCATTCGTGTTGTGAACCCAGAGCTGGGTAGAAGCTGCTTTTGTATTCAGTGTGAGGT
  GGTGTTTACAGACGACTTTGACAACAGTAGAAGTGTACTCAGTGGTGTCTGTGTATCTGAACTATTTAA
  TTTCGTGTTATGTTTATATGCAGAAATATTTATGGATACTACACCAAGTGTTTATTTAATGTTGATAAA
  TATGACTCTTCAGTCGTCAGCCATGGTGTCCTTTCAAAGTGATTCTTTAAGGTCCACTTGAGCAATGAA
  TAGAGTATATTGGAGCTTTCCTGTGGCTAAGAAGAAGAAACATGTCATCCTGTTGGCATCACCAAGCAC
  CTAACTCTTTCTAGGTAATAAAAAGTCAACGGTCACTTCAACGTAAATGTTTAAAACCACTCTTTAGT
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000740.4
• Summary: The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue. Alternative promoter use and alternative splicing results in multiple transcript variants that have different tissue specificities. [provided by RefSeq, Dec 2016]
• Locus ID: 1131
• MW: 75.5