Acid Phosphatase 2 (ACP2) (NM_001131064) Human 3' UTR Clone

CAT#: SC201066

3' UTR clone of acid phosphatase 2 lysosomal (ACP2) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ACP2
• Synonyms: acid phosphatase 2, lysosomal; Acp-2; LAP; OTTMUSP00000015308
• ACCN: NM_001131064
• Insert Size: 160 bp
• Sequence Data:
  Insert Sequence

  >SC201066 3’UTR clone of NM_001131064
  The sequence shown below is from the reference sequence of NM_001131064. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AGAGTGGCCAGCCCTTCCCTGGGGTGGTGAGGGGACAGCTCTGGCCGTAGGCCTGCTGATGCCAGGCTC
  CTTTTCCCGCTGCCTGTTTCCCCGCTTCGCTCTACAGCTGCTGAAGTTCCCGTTGGGCCCATGTCCCCG
  TTATGAGCAGCTGCAGAACGAG
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001131064.1
• Summary: The protein encoded by this gene belongs to the histidine acid phosphatase family, which hydrolyze orthophosphoric monoesters to alcohol and phosphate. This protein is localized to the lysosomal membrane, and is chemically and genetically distinct from the red cell acid phosphatase. Mice lacking this gene showed multiple defects, including bone structure alterations, lysosomal storage defects, and an increased tendency towards seizures. An enzymatically-inactive allele of this gene in mice showed severe growth retardation, hair-follicle abnormalities, and an ataxia-like phenotype. Alternatively spliced transcript variants have been found for this gene. A C-terminally extended isoform is also predicted to be produced by the use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism. [provided by RefSeq, Oct 2017]
• Locus ID: 53
• MW: 5.5