CYP2R1 (NM_024514) Human 3' UTR Clone

CAT#: SC201024

3' UTR clone of cytochrome P450 family 2 subfamily R polypeptide 1 (CYP2R1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CYP2R1
• ACCN: NM_024514
• Insert Size: 712 bp
• Sequence Data:
  Insert Sequence

  >SC201024 3’UTR clone of NM_024514
  The sequence shown below is from the reference sequence of NM_024514. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  CCCTACCTCATCTGTGCTGAAAGACGCTGAAACTGCCTGGGATGTTTTCGGGAACAAGAATGTATATTT
  GCCTTATCCCTGAACTTGGTTTAATCAAATCAATGTGTGTATTAGAATAAAAGTCACAGCATCAAAAAG
  CCAAATGAACACAGATGTGTCTTAAAATAGTACTAGAGCAGAAACAAGCACTAGTCGTGATTGTTTTTC
  AAGATTTTATGACTTCTGGGGTAAAATTCTCTGTTGAGAGACACAAAGGTGCAAATGATGGGTGTTGCT
  TAAAATGCTAAGAAGGGTCATGGACGCCCTAAGCTGAGCATCTTGTTTAGTACCAGGTACTTTCATCCC
  TTCTTGACTCCATCTGCCTTCTGTCCTGGGGGGCCTTGGTTCCAGAAACCAGCTCCCTTCTGATCAGGT
  ATTGTCATTAATAAACTGTGTGGTACCAGTCCAGTTTAATAGAGGACTACAGAAGAAATTAAAACACAA
  ATAAATGAGTTAGGACTATAGAGGTATTTTAGATATGGTGGGAGATGGTAGAAGAAATATGTATTTAAT
  GTAGAGCCTGCCAGTGCTTTCATATCTTGCTTCCTGCTCTGTCTTCATAAGAACTGTGTCATTCTTCTT
  ATAAATGAGGGTCTGTGTGACCCTGAGTGTCCATCTTCATAGCAGTTAAGTAGATAGGACATTACAATA
  ATATATTTAAAATTTAAAATGA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_024514.5
• Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme is a microsomal vitamin D hydroxylase that converts vitamin D into the active ligand for the vitamin D receptor. A mutation in this gene has been associated with selective 25-hydroxyvitamin D deficiency. [provided by RefSeq, Jul 2008]
• Locus ID: 120227
• MW: 27.2