ASAH2 (NM_001143974) Human 3' UTR Clone

CAT#: SC200461

3' UTR clone of N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 (ASAH2) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ASAH2
• Synonyms: BCDase; HNAC1; LCDase; N-CDase; NCDase
• ACCN: NM_001143974
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC200461 3’UTR clone of NM_001143974
  The sequence shown below is from the reference sequence of NM_001143974. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  TCCCCGGCTTTTGAAGTTGTAACTATTTAGTGAAAAGTTGATAGATCATTTAAAGAACAGCTTTACTCT
  CTACACATTATATAAGTGATTTCAAATGAATGTGAACTAGTGAACTACCATGTTGACTTCTATAATCGT
  CCCTGTTTGGGGACAGATAGTTTACTGCTAATGGGGTGGAGGGGTGTGTGTGTGTGTGTGTGTGTGTGT
  GTGTGTGTGTGTGTGTGTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGTTTGTCCCATATATC
  TTGCTCCAGCAGCCATATATCTTGTGGTCTACAGCCTAAAGCATGATTTCCCTTGAAGTCTTGGGGTTG
  TTTAAAGGAGAGTCCCTTCAATATAAAACCTCTGAAATATTAGTGAGAATGGCTCACTAATGTGAACAA
  TGTTTAAATTATTTATTTATATATAGAATTACTGAATATTAGTACTGGGAAAATTTATAGAAATCATCT
  AGTCTTACCCTTCATCTTACATATAAGAAAAATGGTCTTTTCTTCTAATCACATTTACAAAATATGATA
  TAAACCTTGACCATGAATGTATGAGCCTAATTAGAGAAACAGAAAATCAGCATGTCAGTTTTCCTTCAT
  TCAAAATAACATAGTCTTTCTAAGCAGTCATTCTGGAGTTAACATGCCTAGTTCAGAGCCGCTGTGGAT
  GCTCAAATCATTTCTGGAATTGCCTTCAGGACACAATTATGAGAAAGTCAGACTTCCAATATTTTGATC
  ATGCCTTGTGTTTTCCTAAGTGTCCTTATCCCACTGGATTGTGTCTCTTACTCCCAAGACTTATTCCAA
  ATGATTTTTTGACTCTTTTCATTAATCAAATTCACCCCAAACCAGAAAGTTTTGCCATTAGTGTAGATA
  TTAAAAATATTACTGGCTGGGAAAGCACTCCTCAAAGCAGAGGTTCTAAATTAATTTATCTGTTCTTTT
  ACCATTTGCTCACTCACTCACTCATGGACCCATTCAATCATAGAATATTTAAGATTTATATGCCCAGAA
  TTATAATAGTGCCTAGGCCTTGATAATTAGTGAATTTGTACTTGGGGTGAATGTGTAAGTGTCAAAAGG
  TAACTATTATGAAGGAGAAAATAATCGATTTAATATGTAAATTCTGGGGAAGCATTTAAAAAATCAGTA
  ATCTTCAAGTTATATCTTGTGTATCATCTGTGTTGGATACTCTAATTTGGGGGGAAAGAGGCTCCAGAC
  AGTTTGCTAAATACTATTTTGAAAGTTATTTTTGGGCATTGTAAAATACCTTGCTTCATACCCCAAAGA
  ATAAGATATGACAGTCTGACATCGATTGTCTGAAAGTACTAATTGCATTTAAAATTCTTTAAGTTAACT
  ACAACCTCCCAGACTGCAAAGCAAATTTCCAAACCCCAGGTCATACTTTTGTATGTATGTAAAAGCTTT
  AGCATTTTAGGGAAAAAAATCAATCTGTATTTTAAAAATAAACTTTGAGGTTTGAAAATAATAACAATA
  TTCATAATGGTAGTATTAACATCTATTATGTATAACTTAATCTCAACATAATTTATTGAATTAGAATGA
  ACCTGGAACACTTTTTGATGAATTGGTATCCTAGAGGCTGTTTCCTTGGGCATTAACTGCTGGAGGATT
  AGGTAATCAGGGATTCCAAGTGTTGTGATCTTTTTTCTGATGATGCCACAGTCTTCAGTGTAAAAGTTC
  AGAAGCCTAAAAGAACAAGTGATCCTGAAAGTAGCTGATTCCAGACAAGAAAATTATATTAGAACTTAT
  GATATGGTTTTTTAAAGTATTTGTTATTACTCAGAAGGGTTCATGAATCAGTTTCCACTACCTCTATTC
  CTTCTATTGAAGCTTTCCTCAAATCTACTTGTGATTCCTGATAGCTCACTGGCAACTGTACCAAGGTGG
  CCACTGTATCATCTACTGATCCTACTGCACCTAGCACTCTTGGCTCCAGATAACGCACAGAAACCTAT
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001143974.3
• Summary: Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed 11328816]).[supplied by OMIM, Mar 2008]
• Locus ID: 56624
• MW: 77