F(ab')2 Rabbit IgG (H&L) Antibody

CAT#: TA398187

F(ab')2 Rabbit IgG (H&L) Antibody

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: WB: 1:2,000-1:10,000
  IHC: 1:1,000-1:5,000
  ELISA: 1:20,000 - 1:100,000
• Reactivities: Rabbit
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Anti-Rabbit IgG was produced by repeated immunization with Rabbit IgG whole molecule in goat.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin and anti-Goat IgG F(c).
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL - lot specific
• Conjugation: Unconjugated
• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Note: F(ab')2 Rabbit IgG (H&L) Antibody has been tested by ELISA and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.