Mouse IgG2a Antibody Fluorescein Conjugated Pre-adsorbed

CAT#: TA397818

Mouse IgG2a Antibody Fluorescein Conjugated Pre-adsorbed

Product Images

Dot Blot of Goat anti-Mouse IgG2a Antibody Fluorescein Conjugated Pre-absorbed. Antigen: Mouse IgG2a. Load: Lane 1 - 100 ng Lane 2 - 33.3 ng Lane 3 - 11.1 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng. Primary antibody: n/a. Secondary antibody: Goat anti-Mouse IgG2a Antibody Fluorescein Conjugated Pre-absorbed at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB: 1:2,000 - 1:10,000
  IHC: 1:1,000 - 1:5,000
  IF: 1:1,000 - 1:5,000
  FC: 1:200 - 1:500
  FLISA: 1:10,000 - 1:50,000
• Reactivities: Mouse
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Mouse IgG2a heavy chain
• Specificity: MOUSE IgG2a Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Goat Serum, Mouse Serum and Mouse IgG2a. Specificity was confirmed by ELISA at less than 1% cross-reactivity against other mouse heavy or light chain isotypes. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Reconstitution Method: Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL
• Concentration: 1.7 mg/ml - lot specific
• Conjugation: FITC
• Storage: Store anti mouse secondary antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Note: Mouse IgG2a Fluorescein antibody has been tested by dot blot and is available in a variety of formats. FITC Conjugated Secondary Antibodies are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.