Mouse IgG F(c) Antibody Peroxidase Conjugated

CAT#: TA397250

Mouse IgG F(c) Antibody Peroxidase Conjugated

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: WB: 1:1,000 - 1:10,000
  IHC: 1:500 - 1:2,500
  ELISA: 1:10,000 - 1:50,000
• Reactivities: Mouse
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Anti-Mouse IgG F(c) was produced by repeated immunization with mouse IgG F(c) fragment in goat.
• Specificity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against Mouse IgG F(ab).
• Formulation: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Reconstitution Method: Restore with deionized water (or equivalent) - Reconstitution Volume: 2.0 mL
• Concentration: 10.0 mg/mL - lot specific
• Conjugation: HRP
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Note: Mouse IgG F(c) Antibody is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.