Rat IgG (F(ab)2 Fragment) Fc specific Goat Polyclonal Antibody
CAT#: R1414B
Rat IgG (F(ab)2 Fragment) Fc specific goat polyclonal antibody, Biotin
Conjugation: Unconjugated FITC HRP
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Specifications
Product Data | |
Recommended Dilution | Suiatble for use Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Recommended Dilution(s): This product has been assayed against 1.0 µg of Rat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:90,000 of the reconstitution concentration is suggested for this product. |
Reactivities | Rat |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Rat IgG F(c) fragment |
Specificity | This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(ab')2 or Bovine, Horse and Human Serum Proteins. |
Formulation | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01% (w/v) Sodium Azide as preservative. Label: Biotin State: Lyophilized F(ab')2 fragments. Label: N-Hydroxysuccinimide Ester (BAC) Molar radio: 10-20 BAC molecules per Goat IgG molecule. |
Reconstitution Method | Restore with 0.5 ml of deionized water (or equivalent). |
Concentration | lot specific |
Purification | Immunoaffinity chromatography. |
Conjugation | Biotin |
Storage | Store vial at 2-8°C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing. |
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