Human IgG (H+L chain), Fab Fragment Goat Polyclonal Antibody
CAT#: R1388F
Human IgG (H+L chain), Fab Fragment goat polyclonal antibody, FITC
Conjugation: Unconjugated Biotin HRP
Specifications
Product Data | |
Recommended Dilution | Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Recommended Dilutions: FLISA: 1/10,000-1/50,000. Flow Cytometry: 1/500-1/2,500. IF Microscopy: 1/1,000-1/5,000. |
Reactivities | Human |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Human IgG whole molecule |
Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c). |
Formulation | 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) and 0.01% (w/v) Sodium Azide Label: FITC State: Lyophilized Fab fragment Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons) Absorption emission: 495 nm / 528 nm Molar radio: 4.1 moles FITC per mole of Goat IgG Fab |
Reconstitution Method | Restore with 1.0 ml of deionized water or equivalent. |
Concentration | lot specific |
Purification | Immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation |
Conjugation | FITC |
Storage | Store vial at 2-8°C prior to restoration. Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. For extended storage aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing. Dilute only prior to immediate use. |
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