Rabbit IgG (H+L chain) Chicken Polyclonal Antibody
CAT#: R1363F
Rabbit IgG (H+L chain) chicken polyclonal antibody, FITC
Conjugation: Unconjugated Biotin HRP
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Specifications
Product Data | |
Recommended Dilution | Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based Fluorescent assays requiring lot-to-lot consistency. Recommended Dilutions: IF Microscopy: 1/500-1/2,500. |
Reactivities | Rabbit |
Host | Chicken |
Clonality | Polyclonal |
Immunogen | Rabbit IgG whole molecule. |
Specificity | Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Chicken Serum, Rabbit IgG and Rabbit Serum. |
Formulation | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative. Label: FITC State: Lyophilized purified Ig fraction. Label: Highly purified isomer I of Fluorescein isothiocyanate. Care is taken to ensure complete removal of any free Fluorescein from the final product Absorption emission: 495 nm / 528 nm Molar radio: 3.8 moles FITC per mole of Chicken IgG. |
Reconstitution Method | Restore with 1.0 ml of deionized water (or equivalent). |
Concentration | lot specific |
Purification | Prepared from monospecific antiserum by Immunoaffinity Chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. |
Conjugation | FITC |
Storage | Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. This product is photosensitive and should be protected from light. Dilute only prior to immediate use. Avoid repeated freezing and thawing. |
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