Human IgA + IgG + IgM Goat Polyclonal Antibody
CAT#: R1343HRP
Human IgA + IgG + IgM goat polyclonal antibody, HRP
Conjugation: Unconjugated Biotin FITC
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Specifications
Product Data | |
Recommended Dilution | Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/20,000-1/100,000. Western blot: 1/2,000-1/10,000. Immunoprecipitation: 1/1,000-1/5,000. |
Reactivities | Human |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Human IgG, IgA and IgM whole molecules in goat. |
Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum, Human IgG, Human IgA and Human IgM. This reagent is suitable for the detection of all Human immunoglobulin isotypes and chain combinations. |
Formulation | 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 Label: HRP State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (IgG and Protease free) Preservative: 0.01% (w/v) Gentamicin Sulfate Label: Horseradish Peroxidase |
Reconstitution Method | Restore with 1.0 ml of deionized water (or equivalent). |
Concentration | 2.0 mg/ml (by UV absorbance at 280 nm) |
Purification | Immunoaffinity Chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. |
Conjugation | HRP |
Storage | Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing. |
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