Human IgM (Mu chain specific) Goat Polyclonal Antibody
CAT#: R1340HRP
Human IgM (Mu chain specific) goat polyclonal antibody, HRP
Conjugation: Unconjugated Biotin FITC
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Specifications
Product Data | |
Recommended Dilution | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions ELISA: 1/80,000. Western blot: 1/2,000-1/20,000. Immunohistochemistry: 1/500-1/2,500. |
Reactivities | Human |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Human IgM µ (mu) heavy chain |
Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgM and Human Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes. |
Formulation | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml Bovine Serum Albumin (BSA IgG and Protease free) and 0.01% (w/v) Gentamicin Sulfate as preservative. Label: HRP State: Lyophilized purified Ig fraction. Label: Horseradish Peroxidase |
Reconstitution Method | Restore with 1.0 ml of deionized water or equivalent. |
Concentration | lot specific |
Purification | Immunoaffinity chromatography. |
Conjugation | HRP |
Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
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