Hamster IgG (H+L chain) Rabbit Polyclonal Antibody

CAT#: R1327F

Hamster IgG (H+L chain) rabbit polyclonal antibody, FITC

Specifications

Product Data

• Recommended Dilution: Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
  Recommended Dilution(s):
  Flow Cytometry: 1/2,000-1/10,000.
  IF Microscopy: 1/500-1/2,500.
• Reactivities: Hamster
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Hamster IgG whole molecule.
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Hamster IgG and Hamster Serum.
• Formulation: 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 containing 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium azide as preservative.
  Label: FITC
  State: Lyophilized purified Ig fraction
  Label: Flourescence isothiocyanate , Mol Weight 390 daltons
  Absorption emission: 495 nm / 528 nm
  Molar radio: 4.5 moles FITC per mole of Rabbit IgG
• Reconstitution Method: Restore with 1.5 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography using Hamster IgG coupled to agarose.
• Conjugation: FITC
• Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.