Monkey IgG (Fc specific) Goat Polyclonal Antibody

CAT#: AP31438HR-N

Monkey IgG (Fc specific) goat polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, ID, IF, IHC, IP, WB
• Recommended Dilution: Dot blot.
  Immunoblotting.
  ELISA.
  Immunocytochemistry.
  Immunohistochemistry on Paraffin Sections.
  This antibody can be used:
  In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases.
  To identify a specific antigen using a reference antibody of monkey origin known to be of the IgG isotype in the middle layer of the indirect test procedure
  In non-isotopic assay methodology (e.g. ELISA) to measure IgG in monkey serum or other body fluids.
  This immunoconjugate is not pre-diluted. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
  Recommended Working Dilutions:
  Histo- and Cytochemistry: 1/100-1/500.
  ELISA and comparable non-precipitating antibody-binding assays: 1/5000-1/20000.
• Reactivities: Monkey
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Purified normal IgG isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule which expresses strict isotypic (class) specificity. It does not react with any non-Ig protein in monkey serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
  Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Precipitation reactions have been observed with IgG in serum of other old-world monkeys, including Cercopithecus, Cynomolgus and Baboon. The conjugate may also react with IgG of other species as has been observed for chimpanzee and man.
• Formulation: PBS, pH 7.2
  Label: HRP
  State: Lyophilized purified Hyperimmune IgG fraction
  Preservative: None
  Label: Horseradish Peroxidase
  Molar radio: ~1.1
• Reconstitution Method: Restore with 1 ml sterile distilled water
• Purification: DEAE-column Chromatography
• Conjugation: HRP
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one week
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Note: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.