SH3BP1 Mouse Monoclonal Antibody [Clone ID: OTI3G1]

CAT#: TA811446

SH3BP1 mouse monoclonal antibody,clone OTI3G1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SH3BP1 (RC219860, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SH3BP1 (1:2000). Positive lysates LY412861 (100ug) and LC412861 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from cell lines and/or tissue lysates by using anti-SH3BP1 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SH3BP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811446) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SH3BP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811446) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SH3BP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811446) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-SH3BP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811446) (1:2000)

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Specifications

Product Data

• Clone Name: OTI3G1
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:2000
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SH3BP1 (NP_061830) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 75.5 kDa
• Gene Name: SH3 domain binding protein 1
• Database Link:
  NP_061830
  Entrez Gene 23616 Human
  UniProt ID Q9Y3L3
• Synonyms: ARHGAP43